Using morphological attributes for the fast assessment of nutritional responses of Buddhist pine (Podocarpus macrophyllus [Thunb.] D. Don) seedlings to exponential fertilization

Autoři: Liang Xu aff001;  Xie Zhang aff002;  Duhai Zhang aff001;  Hongxu Wei aff003;  Jia Guo aff004
Působiště autorů: Zhejiang Academy of Forestry, Hangzhou, Zhejiang, China aff001;  Hunan Academy of Forestry, Changsha, Hunan, China aff002;  Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, Jilin, China aff003;  Chengbang Ecological Environment Limited Liability Company, Hangzhou, Zhejiang, China aff004
Vyšlo v časopise: PLoS ONE 14(12)
Kategorie: Research Article


Culturing slowly growing tree seedlings is a potential approach for managing the conflict between the increasing demand for ornamental stock and the decreasing area of farmlands due to urbanization. In this study, Buddhist pine (Podocarpus macrophyllus [Thunb.] D. Don) seedlings were raised in multishelves with light-emitting diode lighting in the spectrum of 17:75:8 (red:green:blue) at 190–320 μmol m-2 s-1 with controlled temperature and relative humidity at 19.5°C and 60%, respectively. Seedlings were fed by exponential fertilization (EF) (nitrogen [N]-phosphorus [P]2O5-K2O, 10-7-9) at eight rates of 0 (control), 20 (E20), 40 (E40), 60 (E60), 80 (E80), 100 (E100), 120 (E120), and 140 (E140) mg N seedling-1 for four months through 16 fertilizer applications. The nutritional responses of Buddhist pine seedlings can be identified and classified into various stages in response to increasing doses, up to and over 120 N seedling-1. Morphological traits, i.e., the green color index and leaf area (LA) obtained by digital analysis and the fine root growth, all remained constant in response to doses that induced steady nutrient loading. LA had a positive relationship with most of the nutritional parameters. A dose range between 60 and 120 mg N seedling-1 was recommended for the culture of Buddhist pine seedlings. At this range of fertilizer doses, measuring the leaf area through digital scanning can easily and rapidly indicate the inherent nutrient status of the seedlings.

Klíčová slova:

Buddhism – Fertilizers – Fine roots – Leaves – Nutrients – Pines – Seedlings – Trees


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2019 Číslo 12
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