The transcriptome analysis of the Arabidopsis thaliana in response to the Vibrio vulnificus by RNA-sequencing


Autoři: Yong-Soon Park aff001;  Seon-Kyu Kim aff003;  Seon-Young Kim aff004;  Kyung Mo Kim aff005;  Choong-Min Ryu aff002
Působiště autorů: Biotechnology Research Institute, College of Natural Sciences, Chungbuk National University, Cheongju, South Korea aff001;  Molecular Phytobacteriology Laboratory, Infection Disease Research Center, KRIBB, Daejeon, South Korea aff002;  Personalized Genomic Medicine Research Center, KRIBB, Daejeon, South Korea aff003;  Genome Editing Research Center, KRIBB, Daejeon, South Korea aff004;  Department of Functional Genomics, University of Science and Technology (UST), Daejeon, South Korea aff005;  Microbial Resource Center, KRIBB, Jeongeup, South Korea aff006;  Biosystem and Bioengineering Program, University of Science and Technology (UST), Daejeon, South Korea aff007
Vyšlo v časopise: PLoS ONE 14(12)
Kategorie: Research Article
doi: 10.1371/journal.pone.0225976

Souhrn

Because of the recent increase in the demand for fresh produce, contamination of raw food products has become an issue. Foodborne diseases are frequently caused by the infection of leguminous plants by human bacterial pathogens. Moreover, contamination by Vibrio cholerae, closely related with Vibrio vulnificus, has been reported in plants and vegetables. Here, we investigated the possibility of Vibrio vulnificus 96-11-17M, an opportunistic human pathogen, to infect and colonize Arabidopsis thaliana plants, resulting in typical disease symptoms at 5 and 7 days post-inoculation in vitro and in planta under artificial and favorable conditions, respectively. RNA-Seq analysis revealed 5,360, 4,204, 4,916 and 3,741 differentially expressed genes (DEGs) at 12, 24, 48 and 72 h post-inoculation, respectively, compared with the 0 h time point. Gene Ontology analysis revealed that these DEGs act in pathways responsive to chemical and hormone stimuli and plant defense. The expression of genes involved in salicylic acid (SA)-, jasmonic acid (JA)- and ethylene (ET)-dependent pathways was altered following V. vulnificus inoculation. Genetic analyses of Arabidopsis mutant lines verified that common pathogen-associated molecular pattern (PAMP) receptors perceive the V. vulnificus infection, thus activating JA and ET signaling pathways. Our data indicate that the human bacterial pathogen V. vulnificus 96-11-17M modulates defense-related genes and host defense machinery in Arabidopsis thaliana under favorable conditions.

Klíčová slova:

Arabidopsis thaliana – Gene expression – Leaves – Plant bacterial pathogens – Plant pathology – Population density – Pseudomonas syringae – Vibrio vulnificus


Zdroje

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2019 Číslo 12