Rapid label-free analysis of Opisthorchis viverrini eggs in fecal specimens using confocal Raman spectroscopy

Autoři: Oranat Chuchuen aff001;  Thani Thammaratana aff003;  Oranuch Sanpool aff002;  Rutchanee Rodpai aff002;  Wanchai Maleewong aff002;  Pewpan M. Intapan aff002
Působiště autorů: Department of Chemical Engineering, Faculty of Engineering, Khon Kaen University, Khon Kaen, Thailand aff001;  Research and Diagnostic Center for Emerging Infectious Diseases, Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand aff002;  Research Affairs, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand aff003;  Department of Parasitology and Excellence in Medical Innovation, and Technology Research Group, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand aff004
Vyšlo v časopise: PLoS ONE 14(12)
Kategorie: Research Article
doi: 10.1371/journal.pone.0226762


Opisthorchis viverrini, a human liver fluke, is highly prevalent in Southeast Asia. Definitive diagnosis of infection is usually achieved parasitologically through the discovery of fluke eggs in feces. However, the eggs of O. viverrini are difficult to differentiate morphologically from those of other minute intestinal flukes in fecal samples, even for experienced technicians. The present study developed a label-free optical methodology for analysis of O. viverrini eggs using Raman spectroscopy. Raman features of O. viverrini eggs were reported that can be used as marker bands for the efficient analysis of O. viverrini eggs in fecal specimens. The methodology presented here allows for the rapid detection of O. viverrini egg infection and can be readily and practicably applied in any clinical setting, even those in which a trained parasitologist is not available.

Klíčová slova:

Cancer detection and diagnosis – Fish – Hamsters – Optical materials – Parasitic diseases – Raman spectroscopy – Opisthorchis viverrini – Opisthorchiasis


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Článek vyšel v časopise


2019 Číslo 12