Rapid label-free analysis of Opisthorchis viverrini eggs in fecal specimens using confocal Raman spectroscopy


Autoři: Oranat Chuchuen aff001;  Thani Thammaratana aff003;  Oranuch Sanpool aff002;  Rutchanee Rodpai aff002;  Wanchai Maleewong aff002;  Pewpan M. Intapan aff002
Působiště autorů: Department of Chemical Engineering, Faculty of Engineering, Khon Kaen University, Khon Kaen, Thailand aff001;  Research and Diagnostic Center for Emerging Infectious Diseases, Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand aff002;  Research Affairs, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand aff003;  Department of Parasitology and Excellence in Medical Innovation, and Technology Research Group, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand aff004
Vyšlo v časopise: PLoS ONE 14(12)
Kategorie: Research Article
doi: 10.1371/journal.pone.0226762

Souhrn

Opisthorchis viverrini, a human liver fluke, is highly prevalent in Southeast Asia. Definitive diagnosis of infection is usually achieved parasitologically through the discovery of fluke eggs in feces. However, the eggs of O. viverrini are difficult to differentiate morphologically from those of other minute intestinal flukes in fecal samples, even for experienced technicians. The present study developed a label-free optical methodology for analysis of O. viverrini eggs using Raman spectroscopy. Raman features of O. viverrini eggs were reported that can be used as marker bands for the efficient analysis of O. viverrini eggs in fecal specimens. The methodology presented here allows for the rapid detection of O. viverrini egg infection and can be readily and practicably applied in any clinical setting, even those in which a trained parasitologist is not available.

Klíčová slova:

Cancer detection and diagnosis – Fish – Hamsters – Optical materials – Parasitic diseases – Raman spectroscopy – Opisthorchis viverrini – Opisthorchiasis


Zdroje

1. Sripa B, Kaewkes S, Intapan PM, Maleewong W, Brindley PJ. Food-borne trematodiases in Southeast Asia epidemiology, pathology, clinical manifestation and control. Adv Parasitol 2010; 72: 305–50. doi: 10.1016/S0065-308X(10)72011-X 20624536

2. Zheng S, Zhu Y, Zhao Z, Wu Z, Okanurak K, Lv Z. Liver fluke infection and cholangiocarcinoma: a review. Parasitol Res. 2017; 116: 11–9.

3. IARC Working Group on the Evaluation of Carcinogenic Risks to Humans. Biological agents. Volume 100 B. A review of human carcinogens. IARC Monogr Eval Carcinog Risks Hum. 2012;100:1–441.

4. Fried B, Reddy A, Mayer D. Helminths in human carcinogenesis. Cancer Lett. 2011;305:239–49. doi: 10.1016/j.canlet.2010.07.008 20667649

5. Kaewkes S, Elkins DB, Sithithaworn P, Haswell-Elkins MR. Comparative studies on the morphology of the eggs of Opisthorchis viverrini and lecithodendriid trematodes. Southeast Asian J Trop Med Public Health 1991;22:623–30. 1820653

6. Raman CV. A change of wave-length in light scattering. Nature. 1928;121:619.

7. Chuchuen O, Henderson MH, Sykes C, Kim MS, Kashuba AD, Katz DF. Quantitative analysis of microbicide concentrations in fluids, gels and tissues using confocal Raman spectroscopy. PLoS one. 2013;8(12):e85124. doi: 10.1371/journal.pone.0085124 24386455

8. Chuchuen O, Maher JR, Simons MG, Peters JJ, Wax AP, Katz DF. Label-Free Measurements of Tenofovir Diffusion Coefficients in a Microbicide Gel Using Raman Spectroscopy. J Pharm Sci. 2017;106:639–44. doi: 10.1016/j.xphs.2016.09.030 27837968

9. Chuchuen O, Maher JR, Henderson MH, Desoto M, Rohan LC, Wax A, et al. Label-free analysis of tenofovir delivery to vaginal tissue using co-registered confocal Raman spectroscopy and optical coherence tomography. PloS one. 2017;12:e0185633. doi: 10.1371/journal.pone.0185633 28961280

10. Maher JR, Chuchuen O, Henderson MH, Kim S, Rinehart MT, Kashuba AD, et al. Co-localized confocal Raman spectroscopy and optical coherence tomography (CRS-OCT) for depth-resolved analyte detection in tissue. Biomed Opt Express. 2015;6:2022–35. doi: 10.1364/BOE.6.002022 26114026

11. Presnell AL, Chuchuen O, Simons MG, Maher JR, Katz DF. Full depth measurement of tenofovir transport in rectal mucosa using confocal Raman spectroscopy and optical coherence tomography. Drug Deliv Transl Res. 2018;8:843–52. doi: 10.1007/s13346-018-0495-7 29468424

12. Haka AS, Shafer-Peltier KE, Fitzmaurice M, Crowe J, Dasari RR, Feld MS. Diagnosing breast cancer by using Raman spectroscopy. Proc Natl Acad Sci U S A. 2005;102:12371–6. doi: 10.1073/pnas.0501390102 16116095

13. Surmacki J, Musial J, Kordek R, Abramczyk H. Raman imaging at biological interfaces: applications in breast cancer diagnosis. Mol Cancer. 2013;12:48. doi: 10.1186/1476-4598-12-48 23705882

14. Huang Z, McWilliams A, Lui H, McLean DI, Lam S, Zeng H. Near‐infrared Raman spectroscopy for optical diagnosis of lung cancer. Int J Cancer. 2003;107:1047–52. doi: 10.1002/ijc.11500 14601068

15. Lui H, Zhao J, McLean D, Zeng H. Real-time Raman spectroscopy for in vivo skin cancer diagnosis. Cancer Res. 2012;72:2491–500. doi: 10.1158/0008-5472.CAN-11-4061 22434431

16. Crow P, Stone N, Kendall CA, Uff JS, Farmer JAM, Barr H, et al. The use of Raman spectroscopy to identify and grade prostatic adenocarcinoma in vitro. Br J Cancer. 2003;89:106. doi: 10.1038/sj.bjc.6601059 12838309

17. Crow P, Molckovsky A, Stone N, Uff J, Wilson B, WongKeeSong LM. Assessment of fiberoptic near-infrared Raman spectroscopy for diagnosis of bladder and prostate cancer. Urology. 2005;65:1126–30. doi: 10.1016/j.urology.2004.12.058 15913721

18. Intapan PM, Maleewong W. Opisthorchis viverrini: influence of maternal infection in hamsters on offspring infected with homologous parasite and their IgG antibody response. Exp Parasitol. 2006;113:67–74. doi: 10.1016/j.exppara.2005.12.008 16472806

19. Sukontason KL, Sukontason K, Piangjai S, Pungpak S, Radomyos P. Prevalence of Opisthorchis viverrini infection among villagers harboring Opisthorchis-like eggs. Southeast Asian J Trop Med Public Health. 2001;32 Suppl 2:23–6.

20. Ditrich O, Giboda M, Scholz T, Beer SA. Comparative morphology of eggs of the Haplorchiinae (Trematoda: Heterophyidae) and some other medically important heterophyid and opisthorchiid flukes. Folia Parasitol (Praha). 1992;39:123–32.

21. Sanpool O, Intapan PM, Thanchomnang T, Janwan P, Lulitanond V, Doanh PN, et al. Rapid detection and differentiation of Clonorchis sinensis and Opisthorchis viverrini eggs in human fecal samples using a duplex real-time fluorescence resonance energy transfer PCR and melting curve analysis. Parasitol Res. 2012;111:89–96. doi: 10.1007/s00436-011-2804-7 22246366

22. Tantrawatpan C, Intapan PM, Thanchomnang T, Sanpool O, Janwan P, Lulitanond V, et al. Development of a PCR assay and pyrosequencing for identification of important human fish-borne trematodes and its potential use for detection in fecal specimens. Parasit Vectors. 2014;7:88. doi: 10.1186/1756-3305-7-88 24589167

23. Scatena E, Baiguera S, Del Gaudio C. Raman Spectroscopy and Aptamers for a Label-Free Approach: Diagnostic and Application Tools. J Healthc Eng. 2019: 2815789.


Článek vyšel v časopise

PLOS One


2019 Číslo 12