#PAGE_PARAMS# #ADS_HEAD_SCRIPTS# #MICRODATA#

Comparison of the Panther Fusion and Allplex assays for the detection of respiratory viruses in clinical samples


Autoři: Lola Folgueira aff001;  Noelia Moral aff001;  Consuelo Pascual aff001;  Rafael Delgado aff001
Působiště autorů: Microbiology Department, Hospital Universitario 12 de Octubre, Madrid, Spain aff001;  Biomedical Research Institute imas12, Hospital Universitario 12 de Octubre, Madrid, Spain aff002;  Department of Medicine, School of Medicine, Universidad Complutense, Madrid, Spain aff003
Vyšlo v časopise: PLoS ONE 14(12)
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pone.0226403

Souhrn

Respiratory viral infections are the most frequent clinical syndrome affecting both children and adults, and early detection is fundamental to avoid infection-related risks and reduce the healthcare costs incurred by unnecessary antibiotic treatments. In this study, performance characteristics of two commercial methods, the Panther Fusion® assay (Hologic Inc., San Diego, CA, USA) were compared to Allplex respiratory panels (Seegene, Seoul, South Korea) for the detection of influenza A (Flu A), influenza B (Flu B), respiratory syncytial virus (RSV), parainfluenza virus (PIV), human metapneumovirus (hMPV), rhinovirus (RV) and adenovirus (AdV) targets. A total of 865 specimens collected prospectively and retrospectively were included, and discordant results were further examined using another commercial product, R-GENE respiratory kits (bioMérieux, Marcy l’Etoile, France). There was high agreement between both methods, with 98.6% concordance and a kappa (k) value of 0.9 (95% CI: 0.89–0.92). A specific analysis of both methods for each viral agent demonstrated comparable sensitivity and specificity, both ranging from 0.83 to 1 with good predictive values for the prospective part of the study. Good agreement between both methods was also found for the κ values obtained (ranging from 97.55% to 98.9%), with the lowest for hMPV (k = 0.83, 95% CI: 0.75–0.91) and RV (k = 0.73, 95% CI: 0.65–0.81). Amplification efficiency, measured according to the value of the cycle threshold (Ct) obtained in each of the amplifications in both tests, was significantly better with Panther Fusion for Flu A, Flu B, hMPV and RV. Regarding discordant results, R-GENE showed higher agreement with Panther Fusion-positive specimens (negative for Allplex; n = 28/71, 34.9%) than with Allplex-positive samples (negative for Panther Fusion; n = 7/49, 14.3%). In summary, Panther Fusion proved to be a more efficient fully-automated methodology, requiring shorter hands-on and turnaround times than Allplex.

Klíčová slova:

Adenoviruses – Influenza – Influenza A virus – Influenza B virus – Respiratory infections – Respiratory syncytial virus – Panthers – Human metapneumovirus


Zdroje

1. Mandell LA. Etiologies of Acute Respiratory Tract Infections. Clinical Infectious Diseases 2005; 41: 503–506. doi: 10.1086/432019 16028159

2. Borchers AT, Chang C, Gershwin ME, Gershwin LJ. Respiratory syncytial virus—a comprehensive review. Clin Rev Allergy Immunol 2013; 45: 331–79. doi: 10.1007/s12016-013-8368-9 23575961

3. Edwards KM, Zhu Y, Griffin MR, Weinberg GA, Hall CB, Szilagyi PG, et al. Burden of human metapneumovirus infection in young children. N Engl J Med 2013; 368: 633–43. doi: 10.1056/NEJMoa1204630 23406028

4. Hahn A, Wang W, Jaggi P, Dvorchik I, Ramilo O, Koranyi K, et al. Human metapneumovirus infections are associated with severe morbidity in hospitalized children of all ages. Epidemiol Infect 2013; 141: 2213–23. doi: 10.1017/S0950268812002920 23290557

5. Iuliano AD, Roguski KM, Chang HH, Muscatello DJ, Palekar R, Tempia S, et al. Estimates of global seasonal influenza-associated respiratory mortality: a modelling study. Lancet 2018; 391: 1285–1300. doi: 10.1016/S0140-6736(17)33293-2 29248255

6. Kwon YS, Park SH, Kim MA, Kim HJ, Park JS, Lee MY, et al. Risk of mortality associated with respiratory syncytial virus and influenza infection in adults. BMC Infect Dis 2017; 17: 785. doi: 10.1186/s12879-017-2897-4 29262784

7. Panda S, Mohakud NK, Pena L, Kumar S. Human metapneumovirus: review of an important respiratory pathogen. Int J Infect Dis 2014; 25: 45–52. doi: 10.1016/j.ijid.2014.03.1394 24841931

8. Keske S, Ergonul O, Tutucu F, Karaaslan D, Palaoglu E, Can F. The rapid diagnosis of viral respiratory tract infections and its impact on antimicrobial stewardship programs. Eur J Clin Microbiol Infect Dis 2018; 37: 779–783. doi: 10.1007/s10096-017-3174-6 29332209

9. Mahony JB, Blackhouse G, Babwah J, Smieja M, Buracond S, Chong S, et al. Cost analysis of multiplex PCR testing for diagnosing respiratory virus infections. J Clin Microbiol 2009; 47: 2812–7. doi: 10.1128/JCM.00556-09 19571025

10. Rogan DT, Kochar MS, Yang S, Quinn JV. Impact of Rapid Molecular Respiratory Virus Testing on Real-Time Decision Making in a Pediatric Emergency Department. J Mol Diagn 2017; 19: 460–467. doi: 10.1016/j.jmoldx.2017.01.009 28341587

11. Ginocchio CC, McAdam AJ. Current Best Practices for Respiratory Virus Testing. Journal of Clinical Microbiology 2011; 49 (9 supplement): S44–S48. doi: 10.1128/JCM.00698-11

12. Charlton CL, Babady E, Ginocchio CC, Hatchette TF, Jerris RC, Li Y, et al. Practical Guidance for Clinical Microbiology Laboratories: Viruses Causing Acute Respiratory Tract Infections. Clin Microbiol Rev 2018; 32: e00042–18. doi: 10.1128/CMR.00042-18 30541871

13. Sam SS, Caliendo AM, Ingersoll J, Abdul-Ali D, Hill CE, Kraft CS. Evaluation of Performance Characteristics of Panther Fusion Assays for Detection of Respiratory Viruses from Nasopharyngeal and Lower Respiratory Tract Specimens. J Clin Microbiol 2018; 56: pii: e00787–18. doi: 10.1128/JCM.00787-18

14. Banerjee D, Kanwar N, Hassan F, Essmyer C, Selvarangan R. Comparison of Six Sample-to-Answer Influenza A/B and Respiratory Syncitial Virus Nucleic Acid Amplificaction Assays Using Respiratory Specimens from Children J Clin Microbiol 2018; 56: e00930–18. doi: 10.1128/JCM.00930-18 30185508

15. Lee J, Lee HS, Cho YG, Choi SI, Kim DS. Evaluation of Allplex Respiratory Panel 1/2/3 Multiplex Real-Time PCR Assays for the Detection of Respiratory Viruses with Influenza A Virus subtyping. Ann Lab Med 2018; 38: 46–50. doi: 10.3343/alm.2018.38.1.46 29071818

16. Huh HJ, Kim JY, Kwon HJ, Yun SA, Lee MK, Lee NY, et al. Performance Evaluation of Allplex Respiratory Panels 1, 2, and 3 for Detection of Respiratory Viruses and Influenza A Virus Subtypes. J Clin Microbiol 2017; 55: 479–484. doi: 10.1128/JCM.02045-16 27903601

17. Gimferrer L, Andres C, Rando A, Pinana M, Codina MG, Martin MDC, et al. Evaluation of Seegene Allplex Respiratory Panel 1 kit for the detection of influenza virus and human respiratory syncytial virus. J Clin Virol 2018; 105: 31–34. doi: 10.1016/j.jcv.2018.05.006 29883908

18. Pillet S, Lardeux M, Dina J, Grattard F, Verhoeven P, Le Goff J, et al. Comparative evaluation of six commercial multiplex PCR kits for the diagnosis of respiratory infections. PLoS One. 2013; 8(8):e72174. doi: 10.1371/journal.pone.0072174 24058410

19. Gomez S, Prieto C, Folgueira L. A prospective study to assess the diagnostic performance of the Sofia® Immunoassay for influenza and RSV detection. J Clin Virol 2016; 77:1–4. doi: 10.1016/j.jcv.2016.01.018 26872325

20. Gómez S, Prieto C, Vera C, R Otero J, Folgueira L. Evaluation of a new rapid diagnostic test for the detection of influenza and RSV. Enferm Infecc Microbiol Clin. 2016 34: 298–302. doi: 10.1016/j.eimc.2015.05.007 26139303

21. Hodinka RL. Respiratory RNA Viruses. Microbiol Spectr 2016; 4. doi: 10.1128/microbiolspec.DMIH2-0028-2016

22. Remolina YA, Ulloa MM, Vargas H, Diaz L, Gomez SL, Saavedra A, Sanchez E, Cortes JA. Viral Infection in Adults with Severe Acute Respiratory Infection in Colombia. PLoS One 2015; 10: e0143152. doi: 10.1371/journal.pone.0143152 26576054

23. Mahony JB, Petrich A, Smieja M. Molecular diagnosis of respiratory virus infections. Crit Rev Clin Lab Sci 2011; 48: 217–49. doi: 10.3109/10408363.2011.640976 22185616

24. Cebey-Lopez M, Herberg J, Pardo-Seco J, Gomez-Carballa A, Martinon Torres N, Salas A, et al. Viral co-Infections in pediatric patients hospitalized with lower tract acute respiratory infections. PLoS One 2015; 10: e0136526. doi: 10.1371/journal.pone.0136526 26332375

25. Jacobs SE, Lamson DM, St George K, Walsh TJ. Human rhinoviruses. Clin Microbiol Rev 2013; 26: 135–62. doi: 10.1128/CMR.00077-12 23297263

26. Banerjee S, Sullender WM, Choudekar A, John C, Tyagi V, Fowler K, et al. Detection and genetic diversity of human metapneumovirus in hospitalized children with acute respiratory infections in India. J Med Virol 2011; 83: 1799–810. doi: 10.1002/jmv.22176 21837798


Článek vyšel v časopise

PLOS One


2019 Číslo 12
Nejčtenější tento týden
Nejčtenější v tomto čísle
Kurzy

Zvyšte si kvalifikaci online z pohodlí domova

KOST
Koncepce osteologické péče pro gynekology a praktické lékaře
nový kurz
Autoři: MUDr. František Šenk

Sekvenční léčba schizofrenie
Autoři: MUDr. Jana Hořínková

Hypertenze a hypercholesterolémie – synergický efekt léčby
Autoři: prof. MUDr. Hana Rosolová, DrSc.

Svět praktické medicíny 5/2023 (znalostní test z časopisu)

Imunopatologie? … a co my s tím???
Autoři: doc. MUDr. Helena Lahoda Brodská, Ph.D.

Všechny kurzy
Kurzy Podcasty Doporučená témata Časopisy
Přihlášení
Zapomenuté heslo

Zadejte e-mailovou adresu, se kterou jste vytvářel(a) účet, budou Vám na ni zaslány informace k nastavení nového hesla.

Přihlášení

Nemáte účet?  Registrujte se

#ADS_BOTTOM_SCRIPTS#