Establishment of normative ranges of the healthy human immune system with comprehensive polychromatic flow cytometry profiling

Autoři: John S. Yi aff001;  Marilyn Rosa-Bray aff002;  Janet Staats aff001;  Pearl Zakroysky aff003;  Cliburn Chan aff004;  Melissa A. Russo aff005;  Chelsae Dumbauld aff001;  Scott White aff001;  Todd Gierman aff002;  Kent J. Weinhold aff001;  Jeffrey T. Guptill aff003
Působiště autorů: Department of Surgery, Duke University School of Medicine, Durham, NC, United States of America aff001;  Biomat USA–Grifols Plasma Operations, United States of America aff002;  Duke Clinical Research Institute, Durham, NC, United States of America aff003;  Department of Biostatistics and Bioinformatics, Duke University School of Medicine, Durham, NC, United States of America aff004;  Department of Neurology, Duke University School of Medicine, Durham, NC, United States of America aff005
Vyšlo v časopise: PLoS ONE 14(12)
Kategorie: Research Article
doi: 10.1371/journal.pone.0225512


Existing normative flow cytometry data have several limitations including small sample sizes, incompletely described study populations, variable flow cytometry methodology, and limited depth for defining lymphocyte subpopulations. To overcome these issues, we defined high-dimensional flow cytometry reference ranges for the healthy human immune system using Human Immunology Project Consortium methodologies after carefully screening 127 subjects deemed healthy through clinical and laboratory testing. We enrolled subjects in the following age cohorts: 18–29 years, 30–39, 40–49, and 50–66 and enrolled cohorts to ensure an even gender distribution and at least 30% non-Caucasians. From peripheral blood mononuclear cells, flow cytometry reference ranges were defined for >50 immune subsets including T-cell (activation, maturation, T follicular helper and regulatory T cell), B-cell, and innate cells. We also developed a web tool for visualization of the dataset and download of raw data. This dataset provides the immunology community with a resource to compare and extract data from rigorously characterized healthy subjects across age groups, gender and race.

Klíčová slova:

African American people – B cells – Flow cytometry – Immune system – Lymphocytes – Monocytes – Regulatory T cells – T cells


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2019 Číslo 12