Intracerebroventricular administration of the thyroid hormone analog TRIAC increases its brain content in the absence of MCT8


Autoři: Soledad Bárez-López aff001;  Carmen Grijota-Martínez aff001;  Xiao-Hui Liao aff004;  Samuel Refetoff aff004;  Ana Guadaño-Ferraz aff001
Působiště autorů: Department of Endocrine and Nervous System Pathophysiology, Instituto de Investigaciones Biomédicas Alberto Sols, Consejo Superior de Investigaciones Científicas (CSIC)-Universidad Autónoma de Madrid (UAM), Madrid, Spain aff001;  Center for Biomedical Research on Rare Diseases (Ciberer), Instituto de Salud Carlos III, Madrid, Spain aff002;  Department of Cell Biology, Faculty of Biology, Universidad Complutense de Madrid, Madrid, Spain aff003;  Department of Medicine, The University of Chicago, Chicago, Illinois, United States of America aff004;  Department of Pediatrics, The University of Chicago, Chicago, Illinois, United States of America aff005;  Committee on Genetics, The University of Chicago, Chicago, Illinois, United States of America aff006
Vyšlo v časopise: PLoS ONE 14(12)
Kategorie: Research Article
doi: 10.1371/journal.pone.0226017

Souhrn

Patients lacking the thyroid hormone (TH) transporter MCT8 present abnormal serum levels of TH: low thyroxine and high triiodothyronine. They also have severe neurodevelopmental defects resulting from cerebral hypothyroidism, most likely due to impaired TH transport across the brain barriers. The use of TH analogs, such as triiodothyroacetic acid (TRIAC), that can potentially access the brain in the absence of MCT8 and restore at least a subset of cerebral TH actions could improve the neurological defects in these patients. We hypothesized that direct administration of TRIAC into the brain by intracerebroventricular delivery to mice lacking MCT8 could bypass the restriction at the brain barriers and mediate TH action without causing hypermetabolism. We found that intracerebroventricular administration of therapeutic doses of TRIAC does not increase further plasma triiodothyronine or further decrease plasma thyroxine levels and does not alter TH content in the cerebral cortex. Although TRIAC content increased in the brain, it did not induce TH-mediated actions on selected target genes. Our data suggest that intracerebroventricular delivery of TRIAC has the ability to target the brain in the absence of MCT8 and should be further investigated to address its potential therapeutic use in MCT8 deficiency.

Klíčová slova:

Blood plasma – Brain damage – Central nervous system – Cerebral cortex – Gene expression – Routes of administration – Water resources – Hypothyroidism


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