Screening of differentially expressed immune-related genes from spleen of broilers fed with probiotic Bacillus cereus PAS38 based on suppression subtractive hybridization

Autoři: Jiajun Li aff001;  Wanqiang Li aff001;  Jianzhen Li aff001;  Zhenhua Wang aff002;  Dan Xiao aff001;  Yufei Wang aff001;  Xueqin Ni aff001;  Dong Zeng aff001;  Dongmei Zhang aff001;  Bo Jing aff001;  Lei Liu aff001;  Qihui Luo aff001;  Kangcheng Pan aff001
Působiště autorů: College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province, China aff001;  Branch of Animal Husbandry and Veterinary Medicine, Chengdu Vocational College of Agricultural Science and Technology, Chengdu, Sichuan Province, China aff002
Vyšlo v časopise: PLoS ONE 14(12)
Kategorie: Research Article
doi: 10.1371/journal.pone.0226829


The aim of this study was to construct the spleen differential genes library of broilers fed with probiotic Bacillus cereus PAS38 by suppression subtractive hybridization (SSH) and screen the immune-related genes. Sixty seven-day-old broilers were randomly divided into two groups. The control group was fed with basal diet, and the treated group was fed with basal diet containing Bacillus cereus PAS38 1×106 CFU/g. Spleen tissues were taken and extracted its total RNA at 42 days old, then SSH was used to construct differential gene library and screen immune-related genes. A total of 119 differentially expressed sequence tags (ESTs) were isolated by SSH and 9 immune-related genes were screened out by Gene ontology analysis. Nine differentially expressed genes were identified by qRT-PCR. JCHAIN, FTH1, P2RX7, TLR7, IGF1R, SMAD7, and SLC7A6 were found to be significantly up-regulated in the treated group. Which was consistent with the results of SSH. These findings imply that probiotic Bacillus cereus PAS38-induced differentially expressed genes in spleen might play an important role in the improvement of immunity for broilers, which provided useful information for further understanding of the molecular mechanism of probiotics responsible to affect the poultry immunity.

Klíčová slova:

Bacillus cereus – Gene expression – Genetic screens – Chickens – Polymerase chain reaction – Probiotics – Spleen – Toll-like receptors


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2019 Číslo 12