Vernonia polysphaera Baker: Anti-inflammatory activity in vivo and inhibitory effect in LPS-stimulated RAW 264.7 cells


Autoři: Iara dos Santos da Silva Oliveira aff001;  Aracélio Viana Colares aff002;  Flávia de Oliveira Cardoso aff003;  Carla Junqueira Moragas Tellis aff004;  Maria do Socorro dos Santos Chagas aff004;  Maria Dutra Behrens aff004;  Kátia da Silva Calabrese aff003;  Fernando Almeida-Souza aff003;  Ana Lúcia Abreu-Silva aff005
Působiště autorů: Rede Nordeste de Biotecnologia, Universidade Federal do Maranhão, São Luís, Maranhão, Brazil aff001;  Centro Universitário Dr. Leão Sampaio, Juazeiro do Norte, Ceará, Brazil aff002;  Laboratório de Imunomodulação e Protozoologia, Fundação Oswaldo Cruz, Rio de Janeiro, Rio de Janeiro, Brazil aff003;  Departamento de Produtos Naturais, Farmanguinhos-Fiocruz, Rio de Janeiro, Rio de Janeiro, Brazil aff004;  Pós-graduação em Ciência Animal, Universidade Estadual do Maranhão, São Luís, Maranhão, Brazil aff005
Vyšlo v časopise: PLoS ONE 14(12)
Kategorie: Research Article
doi: 10.1371/journal.pone.0225275

Souhrn

Species of the Vernonia genius are widely distributed across the world. In traditional communities, they are commonly used in popular medicine for the treatment of inflammatory diseases. The objective of the present study was to evaluate the anti-inflammatory activity of Vernonia polysphaera Baker hydroalcoholic extract. A λ-carrageenan-induced paw edema and peritonitis model was established in BALB/c mice. The in vitro activity of the extract was measured on LPS-stimulated RAW 264.7 cells. There was no toxic effect on mice or on the cells treated with the extract. Animals treated with V. polysphaera extract demonstrated inhibition of paw edema in comparison with the untreated animals at all the analyzed doses. In peritonitis, treatment with the extract at a dose of 500 mg/kg resulted in a lower total leukocyte count in the peritoneal fluid and blood and lower levels of IL-1β, IL-6, TNF-α and PGE-2 than the control group. Cells treated with 50 and 100 μg/mL of the extract exhibited lower levels of nitrite and pro-inflammatory cytokine production and lower COX-2, NF-κB expression. The V. polysphaera extract demonstrated an anti-inflammatory effect, interfering with cell migration, reducing pro-inflammatory cytokine levels and COX-2 expression and consequent interference with PGE-2, as well as inhibiting NF-κB transcription.

Klíčová slova:

Cytokines – Inflammation – Inflammatory diseases – Macrophages – Peritonitis – Transcription factors – White blood cells


Zdroje

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