Candida utilis yeast as a functional protein source for Atlantic salmon (Salmo salar L.): Local intestinal tissue and plasma proteome responses


Autoři: Felipe Eduardo Reveco-Urzua aff001;  Mette Hofossæter aff002;  Mallikarjuna Rao Kovi aff003;  Liv Torunn Mydland aff001;  Ragnhild Ånestad aff001;  Randi Sørby aff002;  Charles McLean Press aff002;  Leidy Lagos aff001;  Margareth Øverland aff001
Působiště autorů: Department of Animal and Aquaculture Sciences, Faculty of Biosciences, Norwegian University of Life Sciences, Aas, Norway aff001;  Department of Basic Sciences and Aquatic Medicine, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Oslo, Norway aff002;  Department of Plant Sciences, Faculty of Biosciences, Norwegian University of Life Sciences, Aas, Norway aff003
Vyšlo v časopise: PLoS ONE 14(12)
Kategorie: Research Article
doi: 10.1371/journal.pone.0218360

Souhrn

Microbial ingredients such as Candida utilis yeast are known to be functional protein sources with immunomodulating effects whereas soybean meal causes soybean meal-induced enteritis in the distal intestine of Atlantic salmon (Salmo salar L.). Inflammatory or immunomodulatory stimuli at the local level in the intestine may alter the plasma proteome profile of Atlantic salmon. These deviations can be helpful indicators for fish health and, therefore potential tools in the diagnosis of fish diseases. The present work aimed to identify local intestinal tissue responses and changes in plasma protein profiles of Atlantic salmon fed inactive dry Candida utilis yeast biomass, soybean meal, or combination of soybean meal based diet with various inclusion levels of Candida utilis. A fishmeal based diet was used as control diet. Inclusion of Candida utilis yeast to a fishmeal based diet did not alter the morphology, immune cell population or gene expression of the distal intestine. Lower levels of Candida utilis combined with soybean meal modulated immune cell populations in the distal intestine and reduced the severity of soybean meal-induced enteritis, while higher inclusion levels of Candida utilis were less effective. Changes in the plasma proteomic profile revealed differences between the diets but did not indicate any specific proteins that could be a marker for health or disease. The results suggest that Candida utilis does not alter intestinal morphology or induce major changes in plasma proteome, and thus could be a high-quality alternative protein source with potential functional properties in diets for Atlantic salmon.

Klíčová slova:

Diet – Fish – Gastrointestinal tract – Plasma proteins – Salmon – Soybean – Yeast – Surface-based morphometry


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