Effect of dietary cellulose supplementation on gut barrier function and apoptosis in a murine model of endotoxemia

Autoři: Valentina Di Caro aff001;  Alicia M. Alcamo aff001;  Jessica L. Cummings aff001;  Robert S. B. Clark aff001;  Elizabeth A. Novak aff004;  Kevin P. Mollen aff004;  Michael J. Morowitz aff004;  Rajesh K. Aneja aff001
Působiště autorů: Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA, United States of America aff001;  Department of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA, United States of America aff002;  Division of Pediatric Critical Care Medicine, UPMC Children’s Hospital of Pittsburgh, Pittsburgh, PA, United States of America aff003;  Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA, United States of America aff004;  Division of Pediatric General and Thoracic Surgery, UPMC Children’s Hospital of Pittsburgh, Pittsburgh, PA, United States of America aff005
Vyšlo v časopise: PLoS ONE 14(12)
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pone.0224838


The gut plays a vital role in critical illness, and alterations in the gut structure and function have been reported in endotoxemia and sepsis models. Previously, we have demonstrated a novel link between the diet-induced alteration of the gut microbiome with cellulose and improved outcomes in sepsis. As compared to mice receiving basal fiber (BF) diet, mice that were fed a non-fermentable high fiber (HF) diet demonstrated significant improvement in survival and decreased organ injury in both cecal-ligation and puncture (CLP) and endotoxin sepsis models. To understand if the benefit conferred by HF diet extends to the gut structure and function, we hypothesized that HF diet would be associated with a reduction in sepsis-induced gut epithelial loss and permeability in mice. We demonstrate that the use of dietary cellulose decreased LPS-mediated intestinal hyperpermeability and protected the gut from apoptosis. Furthermore, we noted a significant increase in epithelial cell proliferation, as evidenced by an increase in the percentage of bromodeoxyuridine-positive cells in HF fed mice as compared to BF fed mice. Thus, the use of HF diet is a simple and effective tool that confers benefit in a murine model of sepsis, and understanding the intricate relationship between the epithelial barrier, gut microbiota, and diet will open-up additional therapeutic avenues for the treatment of gut dysfunction in critical illness.

Klíčová slova:

Apoptosis – Cellulose – Diet – Gastrointestinal tract – Microbiome – Mouse models – Permeability – Sepsis


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2019 Číslo 12
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