Heterogeneous root zone salinity mitigates salt injury to Sorghum bicolor (L.) Moench in a split-root system


Autoři: Huawen Zhang aff001;  Runfeng Wang aff002;  Hailian Wang aff002;  Bin Liu aff002;  Mengping Xu aff002;  Yan’an Guan aff002;  Yanbing Yang aff002;  Ling Qin aff002;  Erying Chen aff002;  Feifei Li aff002;  Ruidong Huang aff001;  Yufei Zhou aff001
Působiště autorů: Agronomy College, Shenyang Agricultural University, Shenyang, Liaoning, China aff001;  Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, Shandong, China aff002;  Shandong Engineering Laboratory for Featured Crops, Jinan, Shandong, China aff003
Vyšlo v časopise: PLoS ONE 14(12)
Kategorie: Research Article
doi: 10.1371/journal.pone.0227020

Souhrn

The heterogeneous distribution of soil salinity across the rhizosphere can moderate salt injury and improve sorghum growth. However, the essential molecular mechanisms used by sorghum to adapt to such environmental conditions remain uncharacterized. The present study evaluated physiological parameters such as the photosynthetic rate, antioxidative enzyme activities, leaf Na+ and K+ contents, and osmolyte contents and investigated gene expression patterns via RNA sequencing (RNA-seq) analysis under various conditions of nonuniformly distributed salt. Totals of 5691 and 2047 differentially expressed genes (DEGs) in the leaves and roots, respectively, were identified by RNA-seq under nonuniform (NaCl-free and 200 mmol·L-1 NaCl) and uniform (100 mmol·L-1 and 100 mmol·L-1 NaCl) salinity conditions. The expression of genes related to photosynthesis, Na+ compartmentalization, phytohormone metabolism, antioxidative enzymes, and transcription factors (TFs) was enhanced in leaves under nonuniform salinity stress compared with uniform salinity stress. Similarly, the expression of the majority of aquaporins and essential mineral transporters was upregulated in the NaCl-free root side in the nonuniform salinity treatment, whereas abscisic acid (ABA)-related and salt stress-responsive TF transcripts were more abundant in the high-saline root side in the nonuniform salinity treatment. In contrast, the expression of the DEGs identified in the nonuniform salinity treatment remained virtually unaffected and was even downregulated in the uniform salinity treatment. The transcriptome findings might be supportive of the increased photosynthetic rate, reduced Na+ levels, increased antioxidative capability in the leaves and, consequently, the growth recovery of sorghum under nonuniform salinity stress as well as the inhibited sorghum growth under uniform salinity conditions. The increased expression of salt resistance genes activated in response to the nonuniform salinity distribution implied that the cross-talk between the nonsaline and high-saline sides of the roots exposed to nonuniform salt stress is potentially regulated.

Klíčová slova:

Arabidopsis thaliana – DNA transcription – Gene expression – Leaves – Oryza – Plant resistance to abiotic stress – Salinity – Transcription factors


Zdroje

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