Profusion of G-quadruplexes on both subunits of metazoan ribosomes
Autoři:
Santi Mestre-Fos aff001; Petar I. Penev aff001; John Colin Richards aff001; William L. Dean aff004; Robert D. Gray aff004; Jonathan B. Chaires aff004; Loren Dean Williams aff001
Působiště autorů:
Center for the Origin of Life, Georgia Institute of Technology, Atlanta, Georgia, United States of America
aff001; School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, United States of America
aff002; School of Biological Sciences, Georgia Institute of Technology, Atlanta, Georgia, United States of America
aff003; James Graham Brown Cancer Center, University of Louisville, Louisville, Kentucky, United States of America
aff004
Vyšlo v časopise:
PLoS ONE 14(12)
Kategorie:
Research Article
doi:
https://doi.org/10.1371/journal.pone.0226177
Souhrn
Mammalian and bird ribosomes are nearly twice the mass of prokaryotic ribosomes in part because of their extraordinarily long rRNA tentacles. Human rRNA tentacles are not fully observable in current three-dimensional structures and their conformations remain to be fully resolved. In previous work we identified sequences that favor G-quadruplexes in silico and in vitro in rRNA tentacles of the human large ribosomal subunit. We demonstrated by experiment that these sequences form G-quadruplexes in vitro. Here, using a more recent motif definition, we report additional G-quadruplex sequences on surfaces of both subunits of the human ribosome. The revised sequence definition reveals expansive arrays of potential G-quadruplex sequences on LSU tentacles. In addition, we demonstrate by a variety of experimental methods that fragments of the small subunit rRNA form G-quadruplexes in vitro. Prior to this report rRNA sequences that form G-quadruplexes were confined to the large ribosomal subunit. Our combined results indicate that the surface of the assembled human ribosome contains numerous sequences capable of forming G-quadruplexes on both ribosomal subunits. The data suggest conversion between duplexes and G-quadruplexes in response to association with proteins, ions, or other RNAs. In some systems it seems likely that the integrated population of RNA G-quadruplexes may be dominated by rRNA, which is the most abundant cellular RNA.
Klíčová slova:
Multiple alignment calculation – Oligomers – Ribosomal RNA – Ribosomes – RNA annealing – Sequence alignment – Sequence motif analysis – Chordata
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