Gene expression is associated with virulence in murine macrophages infected with Leptospira spp

Autoři: Erivelto Corrêa de Araújo Junior aff001;  Leandro Encarnação Garcia aff001;  Matheus Janeck Araújo aff001;  Itamar Souza Oliveira-Junior aff002;  Daniel Robert Arnold aff001;  Flavia Lombardi Lopes aff001;  Márcia Marinho aff001
Působiště autorů: Department of Production and Animal Health, São Paulo State University (Unesp), School of Veterinary Medicine, Araçatuba, SP, Brazil aff001;  Department of Surgery, Discipline of Anesthesia, Pain and Intensive Medicine, Federal University of São Paulo, São Paulo, Brazil aff002
Vyšlo v časopise: PLoS ONE 14(12)
Kategorie: Research Article
doi: 10.1371/journal.pone.0225272


Leptospira genus contains species that affect human health with varying degrees of pathogenicity. In this context, we aimed to evaluate the differences in the modulation of host gene expression by strains of Leptospira varying in virulence. Our data showed a high number of differentially expressed transcripts in murine macrophages following 6h of infection. Leptospira infection modulated a set of genes independently of their degree of virulence. However, pathway analysis indicated that Apoptosis, ATM Signaling, and Cell Cycle: G2/M DNA Damage Checkpoint Regulation were exclusively regulated following infection with the virulent strain. Taken together, results demonstrated that species and virulence play a role during host response to Leptospira spp in murine macrophages, which could contribute to understanding the pathogenesis of leptospirosis.

Klíčová slova:

Apoptosis – Cell cycle and cell division – DNA damage – Gene expression – Immune response – Macrophages – Leptospira – Leptospira interrogans


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2019 Číslo 12