Understanding the variability of handheld spectral-domain optical coherence tomography measurements in supine infants

Autoři: Kira L. Wang aff001;  Xi Chen aff002;  Sandra Stinnett aff001;  Vincent Tai aff001;  Katrina P. Winter aff001;  Du Tran-Viet aff001;  Cynthia A. Toth aff002
Působiště autorů: Department of Ophthalmology, Duke University, Durham, North Carolina, United States of America aff001;  Department of Ophthalmology, Division of Vitreoretinal Diseases and Surgery, Duke University, Durham, North Carolina, United States of America aff002;  Department of Biostatistics and Bioinformatics, Duke University, Durham, North Carolina, United States of America aff003;  Department of Biomedical Engineering, Duke University, Durham, North Carolina, United States of America aff004
Vyšlo v časopise: PLoS ONE 14(12)
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pone.0225960



Central foveal thickness (CFT) measurements from optical coherence tomography (OCT) scans provide a precise measure of severity of pathologic changes in the fovea, progress of disease and response to treatment. Although these measures are additionally valuable to assess foveal development in infants, their reproducibility is not known. The goal of this retrospective study is to evaluate the variation and reproducibility of CFT measurements using handheld spectral-domain OCT (hh-SDOCT) in supine infants compared to conventional adult tabletop imaging.


Imaging sessions with multiple macular, volume scans in one eye were selected for analysis from two participant groups: Group 1, 25 imaging sessions from 21 preterm infants without macular edema imaged supine in the nursery using hh-SDOCT (Leica/Bioptigen Envisu C2300, RTP, NC); Group 2, 25 imaging sessions from 25 adults imaged using tabletop Bioptigen SDOCT. For each imaging session, three macular OCT volumes with acceptable image quality were selected for analysis. CFTs were measured using a customized script for automatic segmentation. An expert grader and a typical grader corrected the segmentation lines for the central foveal frame. Coefficient of variations (CV) and intraclass correlation coefficients (ICC) were calculated for graders and systems and compared to the previous literature on OCT reproducibility.


CFT measurements were repeatable and reproducible for both handheld and tabletop SDOCT systems. For handheld, grader ICC (CI) and mean CV were 0.94 (0.90–0.97) and 3.8 (typical) and 0.98 (0.96–0.99) and 2.9 (expert), and for tabletop were 0.91(0.83–0.96) and 2.1 (typical) and 0.92 (0.86–0.96) and 1.9 (expert). Intergrader reproducibility of handheld and tabletop SDOCT systems were ICC(CI) 0.97 (0.95–0.98) and 0.93 (0.89–0.96) respectively, and both are comparable to previously reported reproducibility of tabletop systems.


Handheld SDOCT is a reproducible instrument to measure foveal thicknesses in supine infants. It can be used in clinical research to evaluate foveal changes during retinal development and pathological conditions.

Klíčová slova:

Eyes – Fovea centralis – Choroid – Imaging techniques – Infants – Retina – Tomography


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