Evaluating the effectiveness of HOCl application on odor reduction and earthworm population growth during vermicomposting of food waste employing Eisenia fetida

Autoři: Chanwoo Kim aff001;  Younggu Her aff002;  Yooan Kim aff001;  Chanhoon Jung aff003;  Hangkyo Lim aff004;  Kyo Suh aff001
Působiště autorů: Graduate School of International Agricultural Technology, Seoul National University, Pyeonchang-gun, Gangwon, Republic of Korea aff001;  Department of Agricultural and Biological Engineering, Tropical Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, Homestead, Florida, United States of America aff002;  Interdisciplinary Program in Agricultural and Forest Meteorology, Seoul National University, Seoul, Republic of Korea aff003;  Department of Biology, Notre Dame of Maryland University, Baltimore, Maryland, United States of America aff004;  Institute of Green Bio Science & Technology, Seoul National University, Gangwon, Republic of Korea aff005
Vyšlo v časopise: PLoS ONE 14(12)
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pone.0226229


Vermicomposting has been recommended as an eco-friendly method to transform organic waste into nutrient resources with minimum energy input. However, odor and pest issues associated with this method limit the use of vermicomposting, especially in indoor conditions. This study evaluated the effectiveness of applying hypochlorous acid (HOCl) to deodorize the vermicomposting process and improve the breeding environment for earthworms (Eisenia fetida). The deodorization performance of HOCl was compared by measuring the amount of ammonia (NH3) and amine (R-NH2) released from the decaying process of two types of food waste: HOCl-treated (HTW) waste and non-treated waste (NTW). The total and individual weights of earthworms in the waste treated with HOCl was measured to evaluate the impact on earthworm reproduction after applying HOCl. The results showed that HOCl application could reduce NH3 by 40% and R-NH2 by 80%, and increase the earthworm population size and total weight by up to 29% and 92%, respectively, compared to the control group. These results suggest that HOCl application is potentially an efficient method to control the odor and to boost earthworm reproduction and thus facilitate vermicomposting for improved food waste treatment and environmental quality.

Klíčová slova:

Amines – Ammonia – Earthworms – Gases – Gut bacteria – Organic materials – Population growth – Population size


1. Lim PN, Wu TY, Shyang Sim EY, Lim SL. The potential reuse of soybean husk as feedstock of Eudrilus eugeniae in vermicomposting. Journal of the Science of Food and Agriculture. 2011;91(14):2637–42. doi: 10.1002/jsfa.4504 21725978

2. ME. 2006 Status of Waste Generation and Disposal in Korea. Ministry of Environment (ME); 2007.

3. 2016 Status of Waste Generation and Disposal in Korea [Internet]. Ministry of Environment (ME). 2017.

4. Gebreegziabher T, Oyedun A, Lam K-L, Lee H, Hui C-W. Optimization of MSW Feed for Waste to Energy Practices. Chemical Engineering Transactions. 2012;29:679–84.

5. Graff O. Gewinnung von biomasse aus abfallstoffen durch kultur des kompostregenwurms Eisenia foetida (Savigny 1826). Landbauforschung Völkenrode. 1974;2:137–42.

6. Warman P, AngLopez M. Vermicompost derived from different feedstocks as a plant growth medium. Bioresource Technology. 2010;101(12):4479–83. doi: 10.1016/j.biortech.2010.01.098 20153632

7. Fornes F, Mendoza-Hernández D, García-de-la-Fuente R, Abad M, Belda RM. Composting versus vermicomposting: a comparative study of organic matter evolution through straight and combined processes. Bioresource technology. 2012;118:296–305. doi: 10.1016/j.biortech.2012.05.028 22705537

8. Fernández-Gómez M, Díaz-Raviña M, Romero E, Nogales R. Recycling of environmentally problematic plant wastes generated from greenhouse tomato crops through vermicomposting. International Journal of Environmental Science and Technology. 2013;10(4):697–708.

9. Shak KPY, Wu TY, Lim SL, Lee CA. Sustainable reuse of rice residues as feedstocks in vermicomposting for organic fertilizer production. Environmental Science and Pollution Research. 2014;21(2):1349–59. doi: 10.1007/s11356-013-1995-0 23900949

10. Gómez-Brandón M, Lores M, Domínguez J. Species-specific effects of epigeic earthworms on microbial community structure during first stages of decomposition of organic matter. PloS one. 2012;7(2):e31895. doi: 10.1371/journal.pone.0031895 22363763

11. Pathak A, Singh M, Kumar V. Composting of municipal solid waste: a sustainable waste management technique in Indian cities–A review. International Journal of Current Research. 2011;3(12):339–46.

12. Singh RP, Singh P, Araujo AS, Ibrahim MH, Sulaiman O. Management of urban solid waste: Vermicomposting a sustainable option. Resources, Conservation and Recycling. 2011;55(7):719–29.

13. Wu TY, Lim SL, Lim PN, Shak KPY, editors. Biotransformation of biodegradable solid wastes into organic fertilizers using composting or/and vermicomposting. 17th Conference on Process Integration, Modelling and Optimisation for Energy Saving and Pollution Reduction PRES; 2014.

14. Swati A, Hait S. A comprehensive review of the fate of pathogens during vermicomposting of organic wastes. Journal of environmental quality. 2018;47(1):16–29. doi: 10.2134/jeq2017.07.0265 29415111

15. Cai L, Gong X, Sun X, Li S, Yu X. Comparison of chemical and microbiological changes during the aerobic composting and vermicomposting of green waste. PloS one. 2018;13(11):e0207494. doi: 10.1371/journal.pone.0207494 30475832

16. Mainoo N-OK, Whalen JK, Barrington S. Earthworm abundance related to soil physicochemical and microbial properties in Accra, Ghana. African Journal of Agricultural Research. 2008;3(3):186–94.

17. Ret Michael F. Allen TAZ. The effects of organic amendments on the restoration of a disturbed coastal sage scrub habitat. Restoration Ecology. 1998;6(1):52–8.

18. Azarmi R, Giglou MT, Taleshmikail RD. Influence of vermicompost on soil chemical and physical properties in tomato (Lycopersicum esculentum) field. African Journal of Biotechnology. 2008;7(14).

19. Pathma J, Sakthivel N. Microbial diversity of vermicompost bacteria that exhibit useful agricultural traits and waste management potential. SpringerPlus. 2012;1(1):26.

20. Edwards CA, Arancon NQ, Sherman RL. Vermiculture technology: earthworms, organic wastes, and environmental management: CRC press; 2010.

21. Garg P, Gupta A, Satya S. Vermicomposting of different types of waste using Eisenia foetida: A comparative study. Bioresource technology. 2006;97(3):391–5. doi: 10.1016/j.biortech.2005.03.009 16168639

22. Liesch AM, Weyers SL, Gaskin JW, Das K. Impact of two different biochars on earthworm growth and survival. Annals of Environmental Science. 2010;4(1):1–9.

23. Mohee R, Soobhany N. Comparison of heavy metals content in compost against vermicompost of organic solid waste: past and present. Resources, Conservation and Recycling. 2014;92:206–13.

24. Mao I-F, Tsai C-J, Shen S-H, Lin T-F, Chen W-K, Chen M-L. Critical components of odors in evaluating the performance of food waste composting plants. Science of the total environment. 2006;370(2–3):323–9. doi: 10.1016/j.scitotenv.2006.06.016 16863658

25. Goto K, Kuwayama E, Nozu R, Ueno M, Hayashimoto N. Effect of hypochlorous acid solution on the eradication and prevention of Pseudomonas aeruginosa infection, serum biochemical variables, and cecum microbiota in rats. Experimental animals. 2015:14–0068.

26. Wu S-H, Lin J-F, Jiang R-S. Antibacterial Effect of Hypochlorous Acid Solution on Nasal Discharge from Patients with Chronic Rhinosinusitis. International journal of otolaryngology. 2018;2018.

27. Wang L, Bassiri M, Najafi R, Najafi K, Yang J, Khosrovi B, et al. Hypochlorous acid as a potential wound care agent: part I. Stabilized hypochlorous acid: a component of the inorganic armamentarium of innate immunity. Journal of burns and wounds. 2007;6.

28. Hakim H, Thammakarn C, Suguro A, Ishida Y, Kawamura A, Tamura M, et al. Evaluation of sprayed hypochlorous acid solutions for their virucidal activity against avian influenza virus through in vitro experiments. Journal of Veterinary Medical Science. 2014:14–0413.

29. Jafry AT, Lee C, Kim D, Han G, Sung W-K, Lee J. Development of high concentrated slightly acidic hypochlorous acid generator for food safety. Journal of Mechanical Science and Technology. 2017;31(9):4541–7.

30. Kim Y-R, Nam S-H. Comparison of the preventive effects of slightly acidic HOCl mouthwash and CHX mouthwash for oral diseases. Biomedical Research. 2018;29(8):1718–23.

31. Hao X, Shen Z, Wang J, Zhang Q, Li B, Wang C, et al. In vitro inactivation of porcine reproductive and respiratory syndrome virus and pseudorabies virus by slightly acidic electrolyzed water. The Veterinary Journal. 2013;197(2):297–301. doi: 10.1016/j.tvjl.2013.02.007 23489846

32. Cai B. Role of hypochlorous acid (HOCl) in sodium chloride-induced inhibition of virus replication. 2016.

33. 40 CFR 180.1054 –Calcium hypochlorite; exemptions from the requirement of a tolerance, (2018).

34. 21 CFR 173.315 –Chemicals used in washing or to assist in the peeling of fruits and vegetables, (2018).

35. Majlessi M, Eslami A, Saleh HN, Mirshafieean S, Babaii S. Vermicomposting of food waste: assessing the stability and maturity. Iranian journal of environmental health science & engineering. 2012;9(1):25.

36. Zarrabi M, Mohammadi AA, Al-Musawi TJ, Saleh HN. Using natural clinoptilolite zeolite as an amendment in vermicomposting of food waste. Environmental Science and Pollution Research. 2018;25(23):23045–54. doi: 10.1007/s11356-018-2360-0 29860684

37. Ali U, Sajid N, Khalid A, Riaz L, Rabbani MM, Syed JH, et al. A review on vermicomposting of organic wastes. Environmental Progress & Sustainable Energy. 2015;34(4):1050–62.

38. GF. Feasibility of a Vermicomposting Operation for Food Waste at the Clearfield County Prison. Gannett Fleming (GF)2002.

39. Bari M, Sabina Y, Isobe S, Uemura T, Isshiki K. Effectiveness of electrolyzed acidic water in killing Escherichia coli O157: H7, Salmonella Enteritidis, and Listeria monocytogenes on the surfaces of tomatoes. Journal of food protection. 2003;66(4):542–8. doi: 10.4315/0362-028x-66.4.542 12696675

40. Sheng X, Shu D, Tang X, Zang Y. Effects of slightly acidic electrolyzed water on the microbial quality and shelf life extension of beef during refrigeration. Food Science & Nutrition. 2018;6(7):1975–81.

41. Appelhof M, Olszewski J. Worms Eat My Garbage: How to Set Up and Maintain a Worm Composting System: Compost Food Waste, Produce Fertilizer for Houseplants and Garden, and Educate Your Kids and Family: Storey Publishing; 2017.

42. Thompson S, Lotter C. Conservation of matter in the life sciences. Science Scope. 2014;38(2):57.

43. Albrich JM, McCarthy CA, Hurst JK. Biological reactivity of hypochlorous acid: implications for microbicidal mechanisms of leukocyte myeloperoxidase. Proceedings of the National Academy of Sciences. 1981;78(1):210–4.

44. McKenna SM, Davies K. The inhibition of bacterial growth by hypochlorous acid. Possible role in the bactericidal activity of phagocytes. Biochemical Journal. 1988;254(3):685–92. doi: 10.1042/bj2540685 2848494

45. Ono T, Yamashita K, Murayama T, Sato T. Microbicidal effect of weak acid hypochlorous solution on various microorganisms. Biocontrol science. 2012;17(3):129–33. doi: 10.4265/bio.17.129 23007104

46. Barbieri F, Montanari C, Gardini F, Tabanelli G. Biogenic amine production by lactic acid bacteria: A Review. Foods. 2019;8(1):17.

47. Medina-Sauza RM, Álvarez-Jiménez M, Delhal A, Reverchon F, Blouin M, Guerrero-Analco JA, et al. Earthworms building up soil microbiota, a review. Frontiers in Environmental Science. 2019;7:81.

48. Davidson SK, Stahl DA. Transmission of nephridial bacteria of the earthworm Eisenia fetida. Appl Environ Microbiol. 2006;72(1):769–75. doi: 10.1128/AEM.72.1.769-775.2006 16391117

49. Rayson MS, Altarawneh M, Mackie JC, Kennedy EM, Dlugogorski BZ. Theoretical study of the ammonia− hypochlorous acid reaction mechanism. The Journal of Physical Chemistry A. 2010;114(7):2597–606. doi: 10.1021/jp9088657 20112901

50. Tarade T, Vrček V. Reactivity of amines with hypochlorous acid: Computational study of steric, electronic, and medium effects. International Journal of Quantum Chemistry. 2013;113(7):881–90.

51. Shin SP, Kim MS, Cho SH, Kim JH, Choresca CH Jr, Han JE, et al. Antimicrobial effect of hypochlorous acid on pathogenic microorganisms. 2013.

52. Smith WL, Arnt L, Mellett D. Carriers for hypochlorous acid vapor. Google Patents; 2010.

53. Oxychem. Sodium Chlorite Amine Odor Control. USA: Occidental Chemical Corporation, 2018.

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2019 Číslo 12
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