Protein, dietary fiber, minerals, antioxidant pigments and phytochemicals, and antioxidant activity in selected red morph Amaranthus leafy vegetable

Autoři: Umakanta Sarker aff001;  Shinya Oba aff002
Působiště autorů: Department of Genetics and Plant Breeding, Faculty of Agriculture, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh aff001;  Laboratory of Field Science, Faculty of Applied Biological Sciences, Gifu University, Yanagido, Gifu, Japan aff002
Vyšlo v časopise: PLoS ONE 14(12)
Kategorie: Research Article


Amaranth has two morphological types (morphs), one is red and another is green morph. Red morph amaranth is a marvelous source of nutrients, antioxidant pigments, minerals, and phytochemicals compared to green morph amaranth. For this purpose, we selected 25 red morph genotypes to evaluate in terms of proximate, minerals, antioxidant pigments and phytochemicals and antioxidant activity in RCBD design in three replicates. The leaves of red morph amaranth are an excellent source of dietary fiber, carbohydrates, moisture, and protein. We found remarkable potassium, calcium, magnesium (24.96, 10.13, 30.01 mg g-1), iron, manganese, copper, zinc (1089.19, 243.59, 25.77, 986.61 μg g-1), chlorophyll a, chlorophyll b (31.79, 16.05 mg 100 g-1), β-cyanins, total flavonoids (102.10 RE μg g-1 DW), β-xanthins, betalains (33.30, 33.09, 66.40 μg 100 g-1), carotenoids, total phenolics (172.23 GAE μg g-1 DW), β-carotene (1225.94, 1043.18 μg g-1), vitamin C (955.19 μg g-1), and antioxidant activity (DPPH and ABTS+) (19.97 and 39.09 TEAC μg g-1 DW) in the red morph amaranth leaves. We can select the genotype RA5, RA8, RA18, RA22, and RA25 as antioxidant-enriched red morph amaranth. It revealed that amaranth β-cyanins, phenolics, betalains, flavonoids, β-xanthins, carotenoids, vitamin C, and β-carotene had strong antioxidant activity. These phytochemicals contributed significantly in the antioxidant potentials of red morphs amaranth. Red morph amaranth could be a potential source of nutrients, antioxidant pigments, minerals, and phytochemicals as these compounds scavenged ROS and served as potential antioxidants in our daily diet to attaining nutritional and antioxidant sufficiency.

Klíčová slova:

Antioxidants – Carotenoids – Chlorophyll – Leaves – Phenols – Phytochemicals – Pigments – vitamin C


1. Cai Y, Sun M, Corke H. Antioxidant activity of betalains from plants of the Amaranthaceae. Journal of Agricultural and. Food Chemistry, 2003, 51, 2288–2294. doi: 10.1021/jf030045u 12670172

2. Stintzing FC, Carle R. Betalains–emerging prospects for food scientists. Trends in Food Science and Technology, 2007, 18, 514–525.

3. Esatbeyoglu T, Wagner AE, Schini-Kerth VB, Rimbach G. Betanin- a food colorant with biological activity. Molecular Nutrition and Food Research, 2015, 59, 36–47. doi: 10.1002/mnfr.201400484 25178819

4. Isabelle M, Lee BL, Lim MT, Koh WP, Huang D, Ong CN. Antioxidant activity and profiles of common fruits in Singapore. Food Chemistry, 2010, 123, 77–84.

5. Sarker U, Islam MT, Rabbani MG, Oba S. Genotypic variability for nutrient, antioxidant, yield and yield contributing traits in vegetable amaranth. Journal of Food Agriculture and Environment, 2014, 12, 168–174.

6. Sarker U, Islam MT, Rabbani MG, Oba S. Genotypic diversity in vegetable amaranth for antioxidant, nutrient and agronomic traits. Indian Journal of Genetics and Plant Breeding, 2017, 77, 173–176.

7. Sarker U, Islam MT, Rabbani MG, Oba S. Variability in total antioxidant capacity, antioxidant leaf pigments and foliage yield of vegetable amaranth. Journal of Integrative Agriculture, 2018a, 17, 1145–1153.

8. Sarker U, Islam MT, Rabbani MG, Oba S. Phenotypic divergence in vegetable amaranth for total antioxidant capacity, antioxidant profile, dietary fiber, nutritional and agronomic traits. Acta Agriculturae Scandinavica Section B- Soil and Plant Science, 2018b, 68, 67–76.

9. Sarker U, Islam MT, Rabbani MG, Oba S. Antioxidant leaf pigments and variability in vegetable amaranth. Genetika. 2018c, 50, 209–220.

10. Chakrabarty T, Sarker U, Hasan M, Rahman MM. Variability in mineral compositions, yield and yield contributing traits of stem amaranth (Amaranthus lividus). Genetika, 2018, 50, 995–1010.

11. Sarker U, Islam MT, Rabbani MG, Oba S. Variability, heritability and genetic association in vegetable amaranth (Amaranthus tricolor). Spanish Journal of Agricultural Research, 2015a, 13, 1–8.

12. Sarker U, Islam MT, Rabbani MG, Oba S. Genotype variability in composition of antioxidant vitamins and minerals in vegetable amaranth. Genetika, 2015b, 47, 85–96.

13. Venskutonis PR, Kraujalis P. Nutritional components of amaranth seeds and vegetables: a review on composition, properties, and uses. Comprehensive Review in Food Science and Food Safety, 2013, 12, 381–412.

14. Sarker U, Islam MT, Rabbani MG, Oba S. Genetic variation and interrelationship among antioxidant, quality and agronomic traits in vegetable amaranth. Turkish Journal of Agriculture and Forestry, 2016, 40, 526–535.

15. Sarker U, Oba S. Catalase, superoxide dismutase, and ascorbate-glutathione cycle enzymes confer drought tolerance of Amaranthus tricolor. Scientific Report, 2018d, 8, 16496. doi: 10.1038/s41598-018-34944-0 30405159

16. Sarker U, Oba S. Drought stress enhances nutritional and bioactive compounds, phenolic acids and antioxidant capacity of Amaranthus leafy vegetable. BMC Plant Biology, 2018e, 18, 258.

17. Sarker U, Islam MT, Oba S. Salinity stress accelerates nutrients, dietary fiber, minerals, phytochemicals and antioxidant activity in Amaranthus tricolor leaves. PLOS One, 2018f, 13, 1–18.

18. Sarker U, Oba S. Salinity stress enhances color parameters, bioactive leaf pigments, vitamins, polyphenols, flavonoids and antioxidant activity in selected Amaranthus leafy vegetables. Journal of the Science of Food and Agriculture, 2019a, 99, 2275–2284. doi: 10.1002/jsfa.9423 30324618

19. Sarker U, Oba S. Augmentation of leaf color parameters, pigments, vitamins, phenolic acids, flavonoids and antioxidant activity in selected Amaranthus tricolor under salinity stress. Scientific Report, 2018g, 8, 12349. doi: 10.1038/s41598-018-30897-6 30120319

20. Sarker U, Oba S. Drought stress effects on growth, ROS markers, compatible solutes, phenolics, flavonoids, and antioxidant activity in A. tricolor. Applied Biochemistry and Biotechnology, 2018h, 186, 999–1016.

21. Shukla S, Bhargava A, Chatterjee A, Pandey AC, Mishra B. Diversity in phenotypic and nutritional traits in vegetable amaranth (A. tricolor), a nutritionally underutilized crop. Journal of the Science of Food and Agriculture, 2010, 90, 139–144. doi: 10.1002/jsfa.3797 20355024

22. Rajan S, Markose BL. Horticultural Science Series-6. In Peter KMV. (Ed.), Propagation of horticultural crops. New Delhi, India: New India Publishing Agency. 2007.

23. Repo-Carrasco-Valencia R, Hellstrom JK, Pihlava JM, Mattila PH. Flavonoids and other phenolic compounds in Andean indigenous grains: Quinoa (Chenopodium quinoa), kaniwa (Chenopodium pallidicaule) and kiwicha (Amaranthus caudatus). Food Chemistry, 2010, 120, 128–133.

24. Dusgupta N, De B. Antioxidant activity of some leafy vegetables of India: A comparative study. Food Chemistry, 2007, 101, 471–474.

25. Steffensen SK, Rinnan A, Mortensen AG, Laursen B, Troiani RM, Noellemeyer EJ, et al. Variations in the polyphenol content of seeds of field grown Amaranthus genotypes. Food Chemistry, 2011, 129, 131–138.

26. Sarker U, Oba S. Response of nutrients, minerals, antioxidant leaf pigments, vitamins, polyphenol, flavonoid and antioxidant activity in selected vegetable amaranth under four soil water content. Food Chemistry, 2018i, 252, 72–83. doi: 10.1016/j.foodchem.2018.01.097 29478565

27. Sarker U, Oba S. Antioxidant constituents of three selected red and green color Amaranthus leafy vegetable. Scientific Reports, (2019b), (Accepted). doi: 10.1038/s41598-019-52033-8 31796754

28. Sarker U, Oba S. Nutraceuticals, antioxidant pigments, and phytochemicals in the leaves of Amaranthus spinosus and Amaranthus viridis weedy species. Scientific Reports, (2019c), (Accepted). doi: 10.1038/s41598-019-50977-5

29. Sarker U, Oba S. Nutritional and antioxidant components and antioxidant capacity in green morph Amaranthus leafy vegetable. Scientific Reports, (2019d), (Accepted).

30. Sarker U, Oba S. Nutrients, minerals, antioxidant pigments and phytochemicals, and antioxidant capacity of the leaves of stem amaranth. Scientific Reports, 2019e, (accepted).

31. Sarker U, Oba S. Nutraceuticals, pigments, phytochemical, and radical scavenging capacity in selected Amaranthus leafy vegetables. PLOS One, (2019f), (accepted).

32. Khanam UKS, Oba S. Bioactive substances in leaves of two amaranth species, Amaranthus lividus, and A. hypochondriacus. Canadian Journal of Plant Science, 2013, 93, 47–58.

33. Khanam UKS, Oba S, Yanase E, Murakami Y. Phenolic acids, flavonoids and total antioxidant capacity of selected leafy vegetables. Journal of Functional Foods. 2012, 4, 979–987.

34. Sun H, Mu T, Xi L, Zhang M, Chen J. Sweet potato (Ipomoea batatas L.) leaves as nutritional and functional foods. Food Chemistry, 2014, 156, 380–389. doi: 10.1016/j.foodchem.2014.01.079 24629984

35. Jimenez-Aguilar DM, Grusak MA. Minerals, vitamin C, phenolics, flavonoids and antioxidant activity of Amaranthus leafy vegetables. Journal of. Food Composition and Analysis, 2017, 58, 33–39.

36. Madruga MS, Camara FS. The chemical composition of “Multimistura” as a food supplement. Food Chemistry, 2000, 68, 41–44.

37. Shahidi F, Chavan UD, Bal AK, McKenzie DB. Chemical composition of beach pea (Lathyrus maritimus L.) plant parts. Food Chemistry, 1999, 64, 39–44.

38. Shukla S, Bhargava A, Chatterjee A, Srivastava J, Singh N, Singh SP. Mineral profile and variability in vegetable amaranth (Amaranthus tricolor). Plant Foods for Human. Nutrition, 2006, 61, 23–28. doi: 10.1007/s11130-006-0004-x 16736385

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2019 Číslo 12
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