Protective effect of Platymiscium floribundum Vog. in tree extract on periodontitis inflammation in rats


Autoři: Jordânia M. O. Freire aff001;  Hellíada V. Chaves aff001;  Alrieta H. Teixeira aff002;  Luzia Herminia T. de Sousa aff001;  Isabela Ribeiro Pinto aff001;  José Jackson do N. Costa aff003;  Nayara Alves de Sousa aff004;  Karuza Maria A. Pereira aff005;  Virgínia C. C. Girão aff005;  Vanessa C. S. Ferreira aff006;  João Evangelista de Ávila dos Santos aff007;  Mary Anne S. Lima aff007;  Antônia T. A. Pimenta aff007;  Raquel de C. Montenegro aff008;  Maria Elisabete A. de Moraes aff008;  Vicente de P. T. Pinto aff004;  Gerardo C. Filho aff004;  Mirna M. Bezerra aff004
Působiště autorů: Northeast Biotechnology Network–Ph.D. Program, Federal University of Ceará, Sobral, Ceará, Brazil aff001;  School of Dentistry, Federal University of Ceará, Sobral, Ceará, Brazil aff002;  School of Medicine, University CenterINTA–UNINTA, Sobral, Ceará, Brazil aff003;  School of Medicine, Federal University of Ceará, Sobral, Ceará, Brazil aff004;  Department of Morphology, School of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil aff005;  Postgraduate Program in Morphofunctional Sciences, Department of Morphology, School of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil aff006;  Department of Organic and Inorganic Chemistry, Federal University of Ceará, Fortaleza, Ceará, Brazil aff007;  Drug Research and Development Center (NPDM), Federal University of Ceará, Fortaleza, Ceará, Brazil aff008
Vyšlo v časopise: PLoS ONE 14(11)
Kategorie: Research Article
doi: 10.1371/journal.pone.0223800

Souhrn

Periodontitis is an immuno-inflammatory disease, which can lead to tooth loss. This study aimed to investigate the efficacy of Platymiscium floribundum Vog., a Brazilian tree which has been used in folk medicine as an anti-inflammatory agent, in a pre-clinical trial of periodontitis in rats. Periodontitis was induced by placing a sterilized nylon (3.0) thread ligature around the cervix of the second left upper molar of the rats, which received (per os) P. floribundum extract (0.1, 1 or 10 mg/kg) or vehicle 1h before periodontitis-challenge and once daily during 11 days. Treatment with P. floribundum (10mg/kg) decreased alveolar bone loss, MPO activity nitrite/nitrate levels, oxidative stress, TNF-α, IL1-β, IL-8/CINC-1, and PGE2 gingival levels, and transcription of TNF-α, IL1-β, COX-2, iNOS, RANK, and RANKL genes, while elevated both BALP serum levels and IL-10 gingival levels. The animals did not show signs of toxicity throughout the experimental course. These findings show that P. floribundum has anti-inflammatory and anti-resorptive properties in a pre-clinical trial of periodontitis, representing an interesting biotechnological tool.

Klíčová slova:

Alveolar bone – Cytokines – Inflammation – Nitric oxide – Oxidative stress – Periodontitis – Scanning electron microscopy – Toxicity


Zdroje

1. Hajishengallis G. The inflammophilic character of the periodontitis‐associated microbiota. Mol Oral Microbiol. 2014; 29 (6): 248–257. doi: 10.1111/omi.12065 24976068

2. Trindade F, Oppenheim FG, Helmerhorst EJ, Amado F, Gomes PS, Vitorino R. Uncovering the molecular networks in periodontitis. Proteomics Clin Appl. 2014; 8 (9–10): 748–761. doi: 10.1002/prca.201400028 24828325

3. Sukhtankar L, Kulloli A, Kathariya R, Shetty S. Effect of non-surgical periodontal therapy on superoxide dismutase levels in gingival tissues of chronic periodontitis patients: A clinical and spectophotometric analysis. Dis Markers. 2013; 34 (5): 305–311. doi: 10.3233/DMA-130978 23478273

4. Uslu MÖ, Eltas A, Marakoğlu İ, Dündar S, Şahin K., Özercan İ H. Effects of diode laser application on inflammation and mpo in periodontal tissues in a rat model. J Appl Oral Sci. 2018; 26 (0): e20170266. doi: 10.1590/1678-7757-2017-0266 30020350

5. Buommino E, Scognamiglio M, Donnarumma G, Fiorent A. Recent advances in natural product-based anti-biofilm approaches to control infections. Mini Rev Med Chem. 2014; 14 (14): 1169–1182. doi: 10.2174/1389557515666150101095853 25553429

6. Militão GCG, Dantas INF, Pessoa C, Falcão MJC, Silveira ER, Curi R, et al. Induction of apoptosis by pterocarpans from Platymiscium floribundum in HL-60 human leukemia cells. Life Sci. 2006; 78 (20): 2409–2417. doi: 10.1016/j.lfs.2005.09.044 16316665

7. Cardoso-Lopes EM, Carreira RC, Agripino DG, Torres LMB, Cordeiro I, Bolzani VS, et al. Screening for antifungal, DNA-damaging and anticholinesterasic activities of Brazilian plants from the Atlantic Rainforest: Ilha do Cardoso State Park. Rev. Bras. Farmacogn. 2008; 18: 655–660.

8. Veloso PA, Pimenta ATA, De Sousa FM, Falcão MJC, Gramosa NV, Da Silva JR, et al. New flavonoids and coumarins from Platymiscium floribundum Vogel. J. Braz. Chem. Soc. 2012; 23 (7): 1239–1243. doi: 10.1590/S0103-50532012000700007

9. Bezerra MM, De Lima V, Alencar VB, Vieira IB, Brito GAC, Ribeiro RA, et al. Selective Cyclooxygenase-2 Inhibition Prevents Alveolar Bone Loss. J Periodontol. 2000; 71(6): 1009–1014. doi: 10.1902/jop.2000.71.6.1009 10914805

10. Kuhr A, Popa-Wagner A, Schmoll H, Schwahn C, Kocher T. Observations on experimental marginal periodontitis in rats. J Periodontal Res. 2004; 39 (2): 101–106. doi: 10.1111/j.1600-0765.2004.00710.x 15009517

11. Whitby LG, Moss DW. Analysis of heat inactivation curves of alkaline phosphatase isoenzymes in serum. Clin Chim Acta. 1975; 59: 361–367 doi: 10.1016/0009-8981(75)90012-1 1126023

12. Bradley PP, Christensen RD, Rothstein G. Cellular and extracellular myeloperoxidase in pyogenic inflammation. Blood. 1982; 60 (3): 618–622. 6286012

13. Ding AH, Nathan CF, Stuehr DJ. Release of reactive nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophages. Comparison of activating cytokines and evidence for independent production. J Immunol. 1988; 141(7): 2407–2412. 3139757

14. Beauchamp C, Fridovich I. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem. 1971; 44 (1): 276–287. doi: 10.1016/0003-2697(71)90370-8 4943714

15. Maehly AC. The assay of catalases and peroxidases. Methods Biochem Anal. 1954; 1(0): 357–424.

16. Livak KJ, Schmittgen TD. Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method. Methods. 2001;25: 402–408. doi: 10.1006/meth.2001.1262 11846609

17. La VD, Tanabe S, Bergeron C, Gafner S, Grenier D. Modulation of matrix metalloproteinase and cytokine production by licorice isolates licoricidin and licorisoflavan A: potential therapeutic approach for periodontitis. J Periodontol. 2011; 82 (1): 122–128. doi: 10.1902/jop.2010.100342 20722535

18. Messier C, Epifano F, Genovese S, Grenier D. Licorice and its potential beneficial effects in common oro‐dental diseases. Oral Dis. 2012; 18 (1): 32–39. doi: 10.1111/j.1601-0825.2011.01842.x 21851508

19. Falcão MJC, Pouliquem YBM, Lima MAS, Gramosa NV, Costa-Lotufo LV, Militão GCG, et al. Cytotoxic flavonoids from Platymiscium floribundum. J Nat Prod. 2005; 68 (3): 423–426. doi: 10.1021/np049854d 15787450

20. Whyte MP. Physiological role of alkaline phosphatase explored in hypophosphatasia. Ann N Y Acad Sci. 2010; 1192 (1): 190–200. doi: 10.1111/j.1749-6632.2010.05387.x 20392236

21. Chang PC, Chao YC, Hsiao MH, Chou HS, Jheng YH, Yu XH, et al. Inhibition of Periodontitis Induction Using a Stimuli‐Responsive Hydrogel Carrying Naringin. J Periodontol. 2017; 88 (2): 190–196. doi: 10.1902/jop.2016.160189 27739344

22. Palm F, Lahdentausta L, SORSA T, Tervahartiala T, Gokel P, Buggle F, et al. Biomarkers of periodontitis and inflammation in ischemic stroke: A case-control study. Innate Immun. 2014; 20 (5): 511–518. doi: 10.1177/1753425913501214 24045341

23. Belibasakis GN, Bostanci N. The RANKL-OPG system in clinical periodontology. J Clin Periodontol. 2012; 39 (3): 239–248. doi: 10.1111/j.1600-051X.2011.01810.x 22092994

24. Teixeira AH, Freire JM, de Sousa LH, Parente AT, de Sousa NA, Arriaga A, et al. Stemodia maritima L. extract decreases inflammation, oxidative stress, and alveolar bone loss in an experimental periodontitis rat model. Front Physiol. 2017; 8 (1): 988. doi: 10.3389/fphys.2017.00988 29249988

25. Sánchez GA, Miozza VA, Delgado A, Busch L. Salivary IL‐1β and PGE 2 as biomarkers of periodontal status, before and after periodontal treatment. J Clin Periodontol. 2013; 40 (12): 1112–1117. doi: 10.1111/jcpe.12164 24118119

26. Ribeiro DDSF, de Oliveira Freire JM, Teixeira AH, do Val DR, de Freitas AR, Gomes FI, et al. Tocoyena sellowiana extract decreases bone loss in an experimental model of periodontitis in rats: Putative role for cyclooxygenase-2 and IL-1β inhibition. Biomed Pharmacother. 2018; 98 (1): 863–872. doi: 10.1016/j.biopha.2018.01.011 29571257

27. Azoubel MCF, Sarmento VA, Cangussu V, Azoubel E, Bittencourt S, Cunha FQ, et al. Adjunctive benefits of systemic etoricoxib in non-surgical treatment of aggressive periodontitis: short-term evaluation. J Periodontol. 2008; 79 (9):1719–1725. doi: 10.1902/jop.2008.080019 18771374

28. Sundar NM, Krishnan V, Krishnaraj S, Hemalatha VT, Alam MN. Comparison of the salivary and the serum nitric oxide levels in chronic and aggressive periodontitis: a biochemical study. J Clin Diagn Res. 2013; 7 (6): 1223. doi: 10.7860/JCDR/2013/5386.3068 23905146

29. Martins CS, Leitão RF, Costa DV, Melo IM, Santos GS, Lima V, et al. Topical HPMC/S-nitrosoglutathione solution decreases inflammation and bone resorption in experimental periodontal disease in rats. PLoS One. 2016; 11(4): e0153716. doi: 10.1371/journal.pone.0153716 27116554

30. Van't Hof RJ, Ralston SH. Nitric oxide and bone. Immunology. 2001; 103 (3): 255–261. doi: 10.1046/j.1365-2567.2001.01261.x 11454054

31. Alexandre JTM, Sousa LHT, Lisboa MRP, Furlaneto FA, do Val DR, Marques M, et al. Anti-inflammatory and antiresorptive effects of Calendula officinalis on inflammatory bone loss in rats. Clin Oral Investig. 2018; 22(6): 2175–2185. doi: 10.1007/s00784-017-2308-7 29288400

32. Val DR, Bezerra MM, Silva AA, Pereira KM, Rios LC, Lemos JC, et al. Tephrosia toxicaria Pers. reduces temporomandibular joint inflammatory hypernociception: The involvement of the HO-1 pathway. Eur J Pain. 2014; 18 (9): 1280–1289. doi: 10.1002/j.1532-2149.2014.488.x 24715714

33. Varela-López A, Bullón P, Giampieri F, Quiles J. Non-nutrient, naturally occurring phenolic compounds with antioxidant activity for the prevention and treatment of periodontal diseases. Antioxidants (Basel). 2015; 4 (3): 447–481. doi: 10.3390/antiox4030447 26783837


Článek vyšel v časopise

PLOS One


2019 Číslo 11