Aerobic exercise increases post-exercise exogenous protein oxidation in healthy young males


Autoři: Gerlof A. R. Reckman aff001;  Gerjan J. Navis aff001;  Wim P. Krijnen aff002;  Roel J. Vonk aff003;  Harriët Jager-Wittenaar aff002;  Cees P. van der Schans aff002
Působiště autorů: Department of Internal Medicine, Division of Nephrology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands aff001;  Research Group Healthy Ageing, Allied Health Care and Nursing, Centre of Expertise Healthy Ageing, Hanze University of Applied Sciences, Groningen, the Netherlands aff002;  Department of Cell Biology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands aff003;  Department of Maxillofacial Surgery, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands aff004;  Department of Rehabilitation, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands aff005;  Department of Health Psychology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands aff006
Vyšlo v časopise: PLoS ONE 14(11)
Kategorie: Research Article
doi: 10.1371/journal.pone.0225803

Souhrn

The capacity to utilize ingested protein for optimal support of protein synthesis and lean body mass is described within the paradigm of anabolic competence. Protein synthesis can be stimulated by physical exercise, however, it is not known if physical exercise affects post-exercise protein oxidation. Characterization of the driving forces behind protein oxidation, such as exercise, can contribute to improved understanding of whole body protein metabolism. The purpose of this study is to determine the effect of two levels of aerobic exercise intensity on immediate post-exercise exogenous protein oxidation. Sixteen healthy males with a mean (SD) age of 24 (4) years participated. The subjects’ VO2-max was estimated with the Åstrand cycling test. Habitual dietary intake was assessed with a three-day food diary. Exogenous protein oxidation was measured by isotope ratio mass spectrometry. These measurements were initiated after the ingestion of a 30 g 13C-milk protein test drink that was followed by 330 minutes breath sample collection. On three different days with at least one week in between, exogenous protein oxidation was measured: 1) during rest, 2) after 15 minutes of aerobic exercise at 30% of VO2-max (moderate intensity), and 3) after 15 minutes of aerobic exercise at 60% of VO2-max (vigorous intensity). After vigorous intensity aerobic exercise, 31.8%±8.0 of the 30 g 13C-milk protein was oxidized compared to 26.2%±7.1 during resting condition (p = 0.012), and 25.4%±7.6 after moderate intensity aerobic exercise compared to resting (p = 0.711). In conclusion, exogenous protein oxidation is increased after vigorous intensity aerobic exercise which could be the result of an increased protein turnover rate.

Klíčová slova:

Exercise – Fats – Heart rate – Ingestion – Oxidation – Protein metabolism – Protein synthesis – Aerobic exercise


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PLOS One


2019 Číslo 11