Screening and characterization of long noncoding RNAs involved in the albinism of Ananas comosus var. bracteatus leaves


Autoři: Zhen Lin aff001;  Yingyuan Xiong aff001;  Yanbin Xue aff001;  Meiqin Mao aff001;  Yixuan Xiang aff001;  Yehua He aff002;  Fatima Rafique aff001;  Hao Hu aff001;  Jiawen Liu aff001;  Xi Li aff001;  Lingxia Sun aff001;  Zhuo Huang aff001;  Jun Ma aff001
Působiště autorů: College of Landscape Architecture of Sichuan Agricultural University, Chengdu, Sichuan, China aff001;  Horticultural Biotechnology College of South China Agricultural University, Guangzhou, Guangdong, China aff002
Vyšlo v časopise: PLoS ONE 14(11)
Kategorie: Research Article
doi: 10.1371/journal.pone.0225602

Souhrn

Long noncoding RNAs (lncRNAs) have been reported to play key regulatory roles in plant growth, development, and biotic and abiotic stress physiology. Revealing the mechanism of lncRNA regulation in the albino portions of leaves is important for understanding the development of chimeric leaves in Ananas comosus var. bracteatus. In this study, a total of 3,543 candidate lncRNAs were identified, among which 1,451 were differentially expressed between completely green (CGr) and completely white (CWh) leaves. LncRNAs tend to have shorter transcripts, lower expression levels, and greater expression specificity than protein-coding genes. Predicted lncRNA targets were functionally annotated by the Gene Ontology (GO), Clusters of Orthologous Groups (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. A lncRNA-mRNA interaction network was constructed, and 36 target mRNAs related to chlorophyll metabolism were predicted to interact with 86 lncRNAs. Among these, 25 significantly differentially expressed lncRNAs putatively interacted with 16 target mRNAs. Based on an expression pattern analysis of the lncRNAs and their target mRNAs, the lncRNAs targeting magnesium chelatase subunit H (ChlH), protochlorophyllide oxidoreductase (POR), and heme o synthase (COX10) were suggested as key regulators of chlorophyll metabolism. This study provides the first lncRNA database for A. comosus var. bracteatus and contributes greatly to understanding the mechanism of epigenetic regulation of leaf albinism.

Klíčová slova:

Alternative splicing – Biosynthesis – Gene expression – Chlorophyll – Leaves – Long non-coding RNAs – Messenger RNA – RNA sequencing


Zdroje

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