Genome-wide histone modification profiling of inner cell mass and trophectoderm of bovine blastocysts by RAT-ChIP


Autoři: Tõnis Org aff001;  Kati Hensen aff001;  Rita Kreevan aff001;  Elina Mark aff002;  Olav Sarv aff003;  Reidar Andreson aff004;  Ülle Jaakma aff002;  Andres Salumets aff003;  Ants Kurg aff001
Působiště autorů: Department of Biotechnology, Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia aff001;  Chair of Animal Breeding and Biotechnology, Estonian University of Life Sciences, Tartu, Estonia aff002;  Competence Centre on Health Technologies, Tartu, Estonia aff003;  Department of Bioinformatics, Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia aff004;  Institute of Genomics, University of Tartu, Tartu, Estonia aff005;  Department of Obstetrics and Gynaecology, Institute of Clinical Medicine, University of Tartu, Tartu, Estonia aff006;  Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia aff007;  Department of Obstetrics and Gynaecology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland aff008
Vyšlo v časopise: PLoS ONE 14(11)
Kategorie: Research Article
doi: 10.1371/journal.pone.0225801

Souhrn

Chromatin immunoprecipitation coupled with next-generation sequencing (ChIP-seq) has revolutionized our understanding of chromatin-related biological processes. The method, however, requires thousands of cells and has therefore limited applications in situations where cell numbers are limited. Here we describe a novel method called Restriction Assisted Tagmentation Chromatin Immunoprecipitation (RAT-ChIP) that enables global histone modification profiling from as few as 100 cells. The method is simple, cost-effective and takes a single day to complete. We demonstrate the sensitivity of the method by deriving the first genome-wide maps of histone H3K4me3 and H3K27me3 modifications of inner cell mass and trophectoderm of bovine blastocyst stage embryos.

Klíčová slova:

Blastocysts – Embryos – Gene expression – Gene regulation – Histone modification – Histones – Chromatin – Immunoprecipitation


Zdroje

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