Expression profile of sonic hedgehog signaling-related molecules in basal cell carcinoma

Autoři: Hye Sung Kim aff001;  Young Sil Kim aff001;  Chul Lee aff002;  Myung Soo Shin aff003;  Jae Wang Kim aff004;  Bo Gun Jang aff001
Působiště autorů: Department of Pathology, Jeju National University College of medicine, Jeju, South Korea aff001;  Department of Pathology, Seoul National University College of medicine, Seoul, South Korea aff002;  Department of Plastic Surgery, Jeju National University College of medicine, Jeju, South Korea aff003;  Department of Dermatology, Jeju National University College of medicine, Jeju, South Korea aff004
Vyšlo v časopise: PLoS ONE 14(11)
Kategorie: Research Article
doi: 10.1371/journal.pone.0225511


Basal cell carcinoma (BCC) is the most common human cancer, characterized by aberrant activation of the hedgehog (HH) signaling pathway resulting from mutations in the patched 1 (PTCH1) or smoothened (SMO) genes. In the present study, to uncover the expression profile of HH signaling-related molecules, we thoroughly examined the mRNA and protein expression levels of six molecules including GLI1, GLI2, PTCH1, PTCH2, SHH, and SMO in BCC and various other cutaneous tumors. Real-time PCR analysis demonstrated that BCC showed remarkably enhanced mRNA expression of all HH molecules, except SMO compared to other skin tumors. However, immunohistochemical analysis revealed that only GLI1 protein was specifically upregulated in BCC, while the other HH-related proteins did not show any significant differences between the tumors. Notably, other skin malignancies such as squamous cell carcinoma, sebaceous carcinoma, and malignant melanoma showed no GLI1 expression and there was no difference in GLI1 expression between the BCC subtypes. In addition, GLI1 and GLI2 expression were strongly associated with the hair follicle stem cell markers, LGR4 and LGR5, which are known target genes of the Wnt pathway. Our results suggest that GLI1 has the potential to be a diagnostically useful marker for differentiating BCC from other skin malignancies and an interaction between the HH and Wnt signaling pathways may be involved in the development of BCCs.

Klíčová slova:

Gene expression – Hair follicles – Hedgehog signaling – Immunohistochemistry techniques – Protein expression – Skin tumors – Squamous cell carcinomas – Basal cell carcinomas


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2019 Číslo 11