Antibacterial efficacy of cold atmospheric plasma against Enterococcus faecalis planktonic cultures and biofilms in vitro


Autoři: Felix Theinkom aff001;  Larissa Singer aff001;  Fabian Cieplik aff002;  Sylvia Cantzler aff003;  Hannes Weilemann aff003;  Maximilian Cantzler aff003;  Karl-Anton Hiller aff002;  Tim Maisch aff001;  Julia L. Zimmermann aff004
Působiště autorů: Department of Dermatology, University Hospital Regensburg, Regensburg, Germany aff001;  Department of Conservative Dentistry and Periodontology, University Hospital Regensburg, Regensburg, Germany aff002;  terraplasma GmbH, Garching, Germany aff003;  terraplasma medical GmbH, Garching, Germany aff004
Vyšlo v časopise: PLoS ONE 14(11)
Kategorie: Research Article
doi: 10.1371/journal.pone.0223925

Souhrn

Nosocomial infections have become a serious threat in our times and are getting more difficult to handle due to increasing development of resistances in bacteria. In this light, cold atmospheric plasma (CAP), which is known to effectively inactivate microorganisms, may be a promising alternative for application in the fields of dentistry and dermatology. CAPs are partly ionised gases, which operate at low temperature and are composed of electrons, ions, excited atoms and molecules, reactive oxygen and nitrogen species. In this study, the effect of CAP generated from ambient air was investigated against Enterococcus faecalis, grown on agar plates or as biofilms cultured for up to 72 h. CAP reduced the colony forming units (CFU) on agar plates by > 7 log10 steps. Treatment of 24 h old biofilms of E. faecalis resulted in CFU-reductions by ≥ 3 log10 steps after CAP treatment for 5 min and by ≥ 5 log10 steps after CAP treatment for 10 min. In biofilm experiments, chlorhexidine (CHX) and UVC radiation served as positive controls and were only slightly more effective than CAP. There was no damage of cytoplasmic membranes upon CAP treatment as shown by spectrometric measurements for release of nucleic acids. Thus, membrane damage seems not to be the primary mechanism of action for CAP towards E. faecalis. Overall, CAP showed pronounced antimicrobial efficacy against E. faecalis on agar plates as well as in biofilms similar to positive controls CHX or UVC.

Klíčová slova:

Antimicrobials – Bacterial biofilms – Biofilm culture – Biofilms – Enterococcus faecalis – Nucleic acids – Ultraviolet C – Plasmas


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