A simple way to improve a conventional A/O-MBR for high simultaneous carbon and nutrient removal from synthetic municipal wastewater

Autoři: Dome Adoonsook aff001;  Chang Chia-Yuan aff003;  Aunnop Wongrueng aff001;  Chayakorn Pumas aff005
Působiště autorů: Department of Environmental Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai Thailand aff001;  Research Program in Control of Hazardous Contaminants in Raw Water Resources for Water Scarcity Resilience, Center of Excellence on Hazardous Substance Management (HSM), Bangkok, Thailand aff002;  Department of Environmental Engineering and Science, Chia Nan University of Pharmacy and Science, Tainan, Taiwan aff003;  College of Resource and Environmental Engineering, Wuhan University of Science and Technology, Wuhan, China aff004;  Center of Excellence in Bioresources for Agriculture, Industry and Medicine, Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand aff005
Vyšlo v časopise: PLoS ONE 14(11)
Kategorie: Research Article
doi: 10.1371/journal.pone.0214976


In this study, two anoxic-oxic membrane bioreactor (A/O-MBR) systems, i.e. conventional and biofilm anoxic-oxic-membrane bioreactors (C-A/O-MBR and BF-A/O-MBR, respectively), were operated in parallel under conditions of complete sludge retention for the purposes of comparing system performance and microbial community composition. Moreover, with the microbial communities, comparisons were made between the adhesive stage and the suspended stage. High average removal of COD, NH4+-N and TN was achieved in both systems. However, TP removal efficiency was remarkably higher in BF-A/O-MBR when compared with C-A/O-MBR. TP mass balance analysis suggested that under complete sludge retention, polyurethane sponges that were added into the anoxic tank played a key role in both phosphorus release and accumulation. The qPCR analysis showed that sponge biomass could maintain a higher level of abundance of total bacteria than the suspended sludge. Meanwhile, AOB and denitrifiers were enriched in the suspended sludge but not in the sponge biomass. Results of illumina sequencing reveal that the compacted sponge in BF-A/O-MBR could promote the growth of bacteria involved in nutrient removal and reduce the amount of filamentous and bacterial growth that is related to membrane fouling in the suspended sludge.

Klíčová slova:

Bacteria – Biofilms – Effluent – Nitrification – Phosphates – Shannon index – Species diversity – Sludge


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