Effect of repeated in vivo microCT imaging on the properties of the mouse tibia

Autoři: Sara Oliviero aff001;  Mario Giorgi aff001;  Peter J. Laud aff004;  Enrico Dall’Ara aff001
Působiště autorů: Department of Oncology and Metabolism, University of Sheffield, Sheffield, United Kingdom aff001;  Insigneo Institute for Medicine, University of Sheffield, Sheffield, United Kingdom aff002;  Certara QSP, Certara UK Ltd., Simcyp Division, Sheffield, United Kingdom aff003;  Statistical Services Unit, University of Sheffield, Sheffield, United Kingdom aff004;  MRC Arthritis Research UK Centre for Integrated research into Musculoskeletal Ageing (CIMA), University of Sheffield, Sheffield, United Kingdom aff005
Vyšlo v časopise: PLoS ONE 14(11)
Kategorie: Research Article
doi: 10.1371/journal.pone.0225127


In longitudinal studies, in vivo micro-Computed Tomography (microCT) imaging is used to investigate bone changes over time due to interventions in mice. However, ionising radiation can provoke significant variations in bone morphometric parameters. In a previous study, we evaluated the effect of reducing the integration time on the properties of the mouse tibia measured from microCT images. A scanning procedure (100 ms integration time, 256 mGy nominal radiation dose) was selected as the best compromise between image quality and radiation dose induced on the animal. In this work, the effect of repeated in vivo scans has been evaluated using the selected procedure. The right tibia of twelve female C57BL/6 (six wild type, WT, six ovariectomised, OVX) and twelve BALB/c (six WT, six OVX) mice was scanned every two weeks, starting at week 14 of age. At week 24, mice were sacrificed and both tibiae were scanned. Standard trabecular and cortical morphometric parameters were calculated. The spatial distribution of densitometric parameters (e.g. bone mineral content) was obtained by dividing each tibia in 40 partitions. Stiffness and strength in compression were estimated using homogeneous linear elastic microCT-based micro-Finite Element models. Differences between right (irradiated) and left (non-irradiated control) tibiae were evaluated for each parameter. The irradiated tibiae had higher Tb.Th (+3.3%) and Tb.Sp (+11.6%), and lower Tb.N (-14.2%) compared to non-irradiated tibiae, consistently across both strains and intervention groups. A reduction in Tb.BV/TV (-14.9%) was also observed in the C57BL/6 strain. In the OVX group, a small reduction was also observed in Tt.Ar (-5.0%). In conclusion, repeated microCT scans (at 256 mGy, 5 scans, every two weeks) had limited effects on the mouse tibia, compared to the expected changes induced by bone treatments. Therefore, the selected scanning protocol is acceptable for measuring the effect of bone interventions in vivo.

Klíčová slova:

Bone and joint mechanics – Bone imaging – In vivo imaging – Mechanical properties – Stiffness – Ovariectomy


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Článek vyšel v časopise


2019 Číslo 11