#PAGE_PARAMS# #ADS_HEAD_SCRIPTS# #MICRODATA#

Effect of repeated in vivo microCT imaging on the properties of the mouse tibia


Autoři: Sara Oliviero aff001;  Mario Giorgi aff001;  Peter J. Laud aff004;  Enrico Dall’Ara aff001
Působiště autorů: Department of Oncology and Metabolism, University of Sheffield, Sheffield, United Kingdom aff001;  Insigneo Institute for Medicine, University of Sheffield, Sheffield, United Kingdom aff002;  Certara QSP, Certara UK Ltd., Simcyp Division, Sheffield, United Kingdom aff003;  Statistical Services Unit, University of Sheffield, Sheffield, United Kingdom aff004;  MRC Arthritis Research UK Centre for Integrated research into Musculoskeletal Ageing (CIMA), University of Sheffield, Sheffield, United Kingdom aff005
Vyšlo v časopise: PLoS ONE 14(11)
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pone.0225127

Souhrn

In longitudinal studies, in vivo micro-Computed Tomography (microCT) imaging is used to investigate bone changes over time due to interventions in mice. However, ionising radiation can provoke significant variations in bone morphometric parameters. In a previous study, we evaluated the effect of reducing the integration time on the properties of the mouse tibia measured from microCT images. A scanning procedure (100 ms integration time, 256 mGy nominal radiation dose) was selected as the best compromise between image quality and radiation dose induced on the animal. In this work, the effect of repeated in vivo scans has been evaluated using the selected procedure. The right tibia of twelve female C57BL/6 (six wild type, WT, six ovariectomised, OVX) and twelve BALB/c (six WT, six OVX) mice was scanned every two weeks, starting at week 14 of age. At week 24, mice were sacrificed and both tibiae were scanned. Standard trabecular and cortical morphometric parameters were calculated. The spatial distribution of densitometric parameters (e.g. bone mineral content) was obtained by dividing each tibia in 40 partitions. Stiffness and strength in compression were estimated using homogeneous linear elastic microCT-based micro-Finite Element models. Differences between right (irradiated) and left (non-irradiated control) tibiae were evaluated for each parameter. The irradiated tibiae had higher Tb.Th (+3.3%) and Tb.Sp (+11.6%), and lower Tb.N (-14.2%) compared to non-irradiated tibiae, consistently across both strains and intervention groups. A reduction in Tb.BV/TV (-14.9%) was also observed in the C57BL/6 strain. In the OVX group, a small reduction was also observed in Tt.Ar (-5.0%). In conclusion, repeated microCT scans (at 256 mGy, 5 scans, every two weeks) had limited effects on the mouse tibia, compared to the expected changes induced by bone treatments. Therefore, the selected scanning protocol is acceptable for measuring the effect of bone interventions in vivo.

Klíčová slova:

Bone and joint mechanics – Bone imaging – In vivo imaging – Mechanical properties – Stiffness – Ovariectomy


Zdroje

1. Bouxsein ML, Boyd SK, Christiansen BA, Guldberg RE, Jepsen KJ, Müller R. Guidelines for assessment of bone microstructure in rodents using micro–computed tomography. Journal of Bone and Mineral Research. 2010;25(7):1468–86. doi: 10.1002/jbmr.141 20533309

2. Dall’Ara E, Boudiffa M, Taylor C, Schug D, Fiegle E, Kennerley AJ, et al. Longitudinal imaging of the ageing mouse. Mechanisms of Ageing and Development. 2016;160:93–116. doi: 10.1016/j.mad.2016.08.001 27530773

3. Buie HR, Moore CP, Boyd SK. Postpubertal architectural developmental patterns differ between the L3 vertebra and proximal tibia in three inbred strains of mice. Journal of Bone and Mineral Research. 2008;23(12):2048–59. doi: 10.1359/jbmr.080808 18684086

4. Campbell G, Tiwari S, Grundmann F, Purcz N, Schem C, Glüer C-C. Three-dimensional Image Registration Improves the Long-term Precision of In Vivo Micro-Computed Tomographic Measurements in Anabolic and Catabolic Mouse Models. Calcified Tissue International. 2014;94(3):282–92. doi: 10.1007/s00223-013-9809-4 24170302

5. Lu Y, Boudiffa M, Dall’Ara E, Liu Y, Bellantuono I, Viceconti M. Longitudinal effects of Parathyroid Hormone treatment on morphological, densitometric and mechanical properties of mouse tibia. Journal of the Mechanical Behavior of Biomedical Materials. 2017;75(Supplement C):244–51. https://doi.org/10.1016/j.jmbbm.2017.07.034.

6. Birkhold AI, Razi H, Duda GN, Weinkamer R, Checa S, Willie BM. The Periosteal Bone Surface is Less Mechano-Responsive than the Endocortical. Scientific Reports. 2016;6:23480. doi: 10.1038/srep23480 https://www.nature.com/articles/srep23480#supplementary-information. 27004741

7. Holguin N, Brodt MD, Sanchez ME, Silva MJ. Aging diminishes lamellar and woven bone formation induced by tibial compression in adult C57BL/6. Bone. 2014;65:83–91. doi: 10.1016/j.bone.2014.05.006 24836737

8. Klinck RJ, Campbell GM, Boyd SK. Radiation effects on bone architecture in mice and rats resulting from in vivo micro-computed tomography scanning. Medical Engineering & Physics. 2008;30(7):888–95. doi: 10.1016/j.medengphy.2007.11.004 18249025

9. Laperre K, Depypere M, van Gastel N, Torrekens S, Moermans K, Bogaerts R, et al. Development of micro-CT protocols for in vivo follow-up of mouse bone architecture without major radiation side effects. Bone. 2011;49(4):613–22. doi: 10.1016/j.bone.2011.06.031 21763477

10. Willie BM, Birkhold AI, Razi H, Thiele T, Aido M, Kruck B, et al. Diminished response to in vivo mechanical loading in trabecular and not cortical bone in adulthood of female C57Bl/6 mice coincides with a reduction in deformation to load. Bone. 2013;55(2):335–46. doi: 10.1016/j.bone.2013.04.023 23643681

11. Mustafy T, Benoit A, Londono I, Moldovan F, Villemure I. Can repeated in vivo micro-CT irradiation during adolescence alter bone microstructure, histomorphometry and longitudinal growth in a rodent model? PloS one. 2018;13(11):e0207323–e. doi: 10.1371/journal.pone.0207323 30439999.

12. Oliviero S, Lu Y, Viceconti M, Dall'Ara E. Effect of integration time on the morphometric, densitometric and mechanical properties of the mouse tibia. Journal of Biomechanics. 2017;65(Supplement C):203–11. https://doi.org/10.1016/j.jbiomech.2017.10.026.

13. Sacco SM, Saint C, Longo AB, Wakefield CB, Salmon PL, LeBlanc PJ, et al. Repeated irradiation from micro-computed tomography scanning at 2, 4 and 6 months of age does not induce damage to tibial bone microstructure in male and female CD-1 mice. BoneKEy reports. 2017;6:855–. doi: 10.1038/bonekey.2016.87 28277563.

14. Roberts BC, Giorgi M, Oliviero S, Wang N, Boudiffa M, Dall'Ara E. The longitudinal effects of ovariectomy on the morphometric, densitometric and mechanical properties in the murine tibia: A comparison between two mouse strains. Bone. 2019;127:260–70. doi: 10.1016/j.bone.2019.06.024 31254730

15. Kazakia GJ, Burghardt AJ, Cheung S, Majumdar S. Assessment of bone tissue mineralization by conventional x-ray microcomputed tomography: comparison with synchrotron radiation microcomputed tomography and ash measurements. MED PHYS. 2008;35(7):3170–9. doi: 10.1118/1.2924210 18697542

16. Lu Y, Boudiffa M, Dall'Ara E, Bellantuono I, Viceconti M. Development of a protocol to quantify local bone adaptation over space and time: Quantification of reproducibility. Journal of Biomechanics. 2016;(49):2095–9.

17. Oliviero S, Giorgi M, Dall'Ara E. Validation of finite element models of the mouse tibia using digital volume correlation. Journal of the Mechanical Behavior of Biomedical Materials. 2018;86:172–84. doi: 10.1016/j.jmbbm.2018.06.022 29986291

18. Birkhold AI, Razi H, Duda GN, Weinkamer R, Checa S, Willie BM. The influence of age on adaptive bone formation and bone resorption. Biomaterials. 2014;35(34):9290–301. doi: 10.1016/j.biomaterials.2014.07.051 25128376

19. Meijering EHW. Spline interpolation in medical imaging: comparison with other convolution-based approaches. 10th European Signal Processing Conference, Tampere. 2000:1–8. http://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=7075214&isnumber=7065137.

20. Chen Y, Dall׳Ara E, Sales E, Manda K, Wallace R, Pankaj P, et al. Micro-CT based finite element models of cancellous bone predict accurately displacement once the boundary condition is well replicated: A validation study. Journal of the Mechanical Behavior of Biomedical Materials. 2017;65:644–51. doi: 10.1016/j.jmbbm.2016.09.014 27741494

21. Christiansen BA. Effect of micro-computed tomography voxel size and segmentation method on trabecular bone microstructure measures in mice. Bone Reports. 2016;5:136–40. doi: 10.1016/j.bonr.2016.05.006 27430011

22. Webster DJ, Morley PL, van Lenthe GH, Müller R. A novel in vivo mouse model for mechanically stimulated bone adaptation–a combined experimental and computational validation study. Computer Methods in Biomechanics and Biomedical Engineering. 2008;11(5):435–41. doi: 10.1080/10255840802078014 18612871

23. Pistoia W, van Rietbergen B, Lochmüller EM, Lill CA, Eckstein F, Rüegsegger P. Estimation of distal radius failure load with micro-finite element analysis models based on three-dimensional peripheral quantitative computed tomography images. Bone. 2002;30(6):842–8. doi: 10.1016/s8756-3282(02)00736-6 12052451

24. Bayraktar HH, Morgan EF, Niebur GL, Morris GE, Wong EK, Keaveny TM. Comparison of the elastic and yield properties of human femoral trabecular and cortical bone tissue. Journal of Biomechanics. 2004;37(1):27–35. doi: 10.1016/s0021-9290(03)00257-4 14672565

25. Judex S, Chung H, Torab A, Xie L, Rubin C, Donahue LR, et al., editors. Micro-CT induced radiation does not exacerbate disuse related bone loss2005 20052005.

26. Margolis DS, Lien Y-HH, Lai L-W, Szivek JA. Bilateral symmetry of biomechanical properties in mouse femora. Medical Engineering & Physics. 2004;26(4):349–53. https://doi.org/10.1016/j.medengphy.2003.11.002.

27. Main RP, Lynch ME, van der Meulen MCH. Load-induced changes in bone stiffness and cancellous and cortical bone mass following tibial compression diminish with age in female mice. The Journal of Experimental Biology. 2014;217(Pt 10):1775–83. doi: 10.1242/jeb.085522 24577445


Článek vyšel v časopise

PLOS One


2019 Číslo 11
Nejčtenější tento týden
Nejčtenější v tomto čísle
Kurzy

Zvyšte si kvalifikaci online z pohodlí domova

KOST
Koncepce osteologické péče pro gynekology a praktické lékaře
nový kurz
Autoři: MUDr. František Šenk

Sekvenční léčba schizofrenie
Autoři: MUDr. Jana Hořínková

Hypertenze a hypercholesterolémie – synergický efekt léčby
Autoři: prof. MUDr. Hana Rosolová, DrSc.

Svět praktické medicíny 5/2023 (znalostní test z časopisu)

Imunopatologie? … a co my s tím???
Autoři: doc. MUDr. Helena Lahoda Brodská, Ph.D.

Všechny kurzy
Kurzy Podcasty Doporučená témata Časopisy
Přihlášení
Zapomenuté heslo

Zadejte e-mailovou adresu, se kterou jste vytvářel(a) účet, budou Vám na ni zaslány informace k nastavení nového hesla.

Přihlášení

Nemáte účet?  Registrujte se

#ADS_BOTTOM_SCRIPTS#