Occurrence and multilocus genotyping of Giardia duodenalis from post-weaned dairy calves in Sichuan province, China


Autoři: Jiaming Dan aff001;  Xueping Zhang aff001;  Zhihua Ren aff001;  Liqin Wang aff002;  Suizhong Cao aff001;  Liuhong Shen aff001;  Junliang Deng aff001;  Zhicai Zuo aff001;  Shumin Yu aff001;  Ya Wang aff001;  Xiaoping Ma aff001;  Haifeng Liu aff001;  Ziyao Zhou aff001;  Yanchun Hu aff001;  Hualin Fu aff001;  Changliang He aff001;  Yi Geng aff001;  Xiaobin Gu aff001;  Guangneng Peng aff001;  Zhijun Zhong aff001
Působiště autorů: College of Veterinary Medicine, Sichuan Agricultural University, Key Laboratory of Animal Disease and Human Health of Sichuan, Chengdu, China aff001;  The Chengdu Zoo, Institute of Wild Animals, Chengdu, China aff002
Vyšlo v časopise: PLoS ONE 14(11)
Kategorie: Research Article
doi: 10.1371/journal.pone.0224627

Souhrn

Giardia duodenalis is a zoonotic parasitic protist and poses a threat to human and animal health. This study investigated the occurrence of G. duodenalis infection in post-weaned calves from Sichuan province, China. Faecal samples were collected from a total of 306 post-weaned calves (3–12 months old) from 10 farms, including 4 intensive feeding farms and 6 free-ranging farms. The overall infection rate of G. duodenalis was 41.2% (126/306) based on the PCR results at any of the three genetic loci: beta-giardin (bg), triose-phosphate isomerase (tpi) and glutamate dehydrogenase (gdh) genes. Giardia duodenalis assemblages E (n = 115, 91.3%), A (n = 3, 2.4%), and A mixed with E (n = 8, 6.3%) were identified among the 126 positive specimens. Multilocus sequence typing of G. duodenalis revealed 34 assemblage E multilocus genotypes (MLGs), 1 assemblage A MLG and 7 mixed assemblage (A and E) MLGs. The eBURST data showed a high degree of genetic diversity within assemblage E MLGs. The phylogenetic tree revealed that MLG E3 was the primary MLG subtype in Sichuan province and also the most widely distributed in China.

Klíčová slova:

DNA extraction – Farms – Genetic loci – China – Phylogenetic analysis – Polymerase chain reaction – Zoonoses – Giardia


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2019 Číslo 11