Microdissection and whole chromosome painting confirm karyotype transformation in cryptic species of the Lariophagus distinguendus (Förster, 1841) complex (Hymenoptera: Pteromalidae)


Autoři: Vladimir E. Gokhman aff001;  Marcelo de Bello Cioffi aff002;  Christian König aff004;  Marie Pollmann aff004;  Cornelia Gantert aff004;  Lars Krogmann aff004;  Johannes L. M. Steidle aff004;  Nadezda Kosyakova aff003;  Thomas Liehr aff003;  Ahmed Al-Rikabi aff003
Působiště autorů: Botanical Garden, Moscow State University, Moscow, Russia aff001;  Department of Genetics and Evolution, Federal University of São Carlos, São Carlos, São Paulo, Brazil aff002;  Institute of Human Genetics, Jena University Hospital, Jena, Germany aff003;  Institute for Zoology, University of Hohenheim, Stuttgart, Germany aff004;  Department of Entomology, State Museum of Natural History Stuttgart, Stuttgart, Germany aff005
Vyšlo v časopise: PLoS ONE 14(11)
Kategorie: Research Article
doi: 10.1371/journal.pone.0225257

Souhrn

Karyotypes of two cryptic species of parasitoid Hymenoptera with n = 5 and 6 belonging to the Lariophagus distinguendus (Förster, 1841) complex, which includes cosmopolitan parasitoids of coleopteran stored-product pests, were studied using glass-needle based microdissection, reverse and cross-species fluorescence in situ hybridisation (FISH). This experiment strongly indicates that the largest metacentric chromosome in the karyotype with n = 5 originated from a particular fusion between the only acrocentric and a smaller metacentric chromosome of the set with n = 6, therefore confirming our previous hypothesis based on the karyotypic analysis using chromosome morphometrics. This study represents the first successful application of both microdissection and whole chromosome painting for the reconstruction of karyotypic rearrangements in closely related species of parasitoids, as well as in the order Hymenoptera in general.

Klíčová slova:

Cryptic speciation – Fluorescent in situ hybridization – Hymenoptera – Chromosome pairs – In situ hybridization – Karyotypes – Morphometry – Polymerase chain reaction


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2019 Číslo 11