Comparative analysis of the accelerated aged seed transcriptome profiles of two maize chromosome segment substitution lines


Autoři: Li Li aff001;  Feng Wang aff001;  Xuhui Li aff001;  Yixuan Peng aff001;  Hongwei Zhang aff002;  Stefan Hey aff003;  Guoying Wang aff002;  Jianhua Wang aff001;  Riliang Gu aff001
Působiště autorů: Seed Science and Technology Research Center, Beijing Innovation Center for Seed Technology (MOA), Beijing Key Laboratory for Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural University, Beijing, China aff001;  Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China aff002;  Department of Agronomy, Iowa State University, Ames, Iowa, United States of America aff003
Vyšlo v časopise: PLoS ONE 14(11)
Kategorie: Research Article
doi: 10.1371/journal.pone.0216977

Souhrn

Seed longevity is one of the most essential characteristics of seed quality. Two chromosome segment substitution lines, I178 and X178, which show significant differences in seed longevity, were subjected to transcriptome sequencing before and after five days of accelerated aging (AA) treatments. Compared to the non-aging treatment, 286 and 220 differentially expressed genes (DEGs) were identified after 5 days of aging treatment in I178 and X178, respectively. Of these DEGs, 98 were detected in both I178 and X178, which were enriched in Gene Ontology (GO) terms of the cellular component of the nuclear part, intracellular part, organelle and membrane. Only 86 commonly downregulated genes were enriched in GO terms of the carbohydrate derivative catabolic process. Additionally, transcriptome analysis of alternative splicing (AS) events in I178 and X178 showed that 63.6% of transcript isoforms occurred AS in all samples, and only 1.6% of transcript isoforms contained 169 genes that exhibited aging-specific AS arising after aging treatment. Combined with the reported QTL mapping result, 7 DEGs exhibited AS after aging treatment, and 13 DEGs in mapping interval were potential candidates that were directly or indirectly related to seed longevity.

Klíčová slova:

Carbohydrates – Energy metabolism – Gene expression – Maize – Nutrient and storage proteins – Seed germination – Seeds – Intracellular membranes


Zdroje

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2019 Číslo 11