Genome-wide identification and characterization, phylogenetic comparison and expression profiles of SPL transcription factor family in B. juncea (Cruciferae)

Autoři: Jian Gao aff001;  Hua Peng aff003;  Fabo Chen aff001;  Yi Liu aff001;  Baowei Chen aff001;  Wenbo Li aff001
Působiště autorů: Department of Life Sciences and Technology, Yangtze Normal University, Fuling, Chongqing, China aff001;  Centre for Green Development and Collaborative Innovation in Wuling Mountain Region, Yangtze Normal University, Fuling, Chongqing, China aff002;  Sichuan Tourism College, Chengdu, Sichuan, China aff003
Vyšlo v časopise: PLoS ONE 14(11)
Kategorie: Research Article
doi: 10.1371/journal.pone.0224704


SQUAMOSA promoter-binding protein-like (SPL), as plant specific transcription factors, is involved in many plant growth and development processes. However, there is less systematical study for SPL transcription factor in B. juncea (Cruciferae). Here, a total of 59 SPL genes classified into eight phylogenetic groups were identified in B. juncea, highly conserved within each ortholog were also found based on gene structure, conserved motif, as well as clustering level. In addition, clustering of SPL domain showed that two zinc finger-like structures and NLS segments were identified in almost of BjuSPLs. Analyzed of putative cis-elements for BjuSPLs demonstrated that SPL transcription factors were involved in adverse environmental changes, such as light, plant stresses and phytohormones response. Expression analysis showed that differentially expressed SPL genes were identified in flower and stem development of Cruciferae; such as BjuSPL3a-B, BjuSPL2b_B and BjuSPL2c_A were significantly expressed in flower; BjuSPL 3b_B and BjuSPL10a_A were significantly expressed in stem node (VP: vegetative period). Moreover, 28 of the 59 BjuSPLs were found involved in their posttranscriptional regulation targeted by miR156. We demonstrated that miR156 negatively regulated BjuSPL10a_A and BjuSPL3b_B to act for stem development in B. juncea.

Klíčová slova:

Arabidopsis thaliana – Introns – Phylogenetic analysis – Protein domains – Protein structure comparison – Seedlings – Sequence motif analysis – Transcription factors


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2019 Číslo 11