Comparison of the inoculum size effects of antibiotics on IMP-6 β-lactamase-producing Enterobacteriaceae co-harboring plasmid-mediated quinolone resistance genes
Autoři:
Yoshihiko Ogawa aff001; Ryuichi Nakano aff002; Kei Kasahara aff001; Tomoki Mizuno aff002; Nobuyasu Hirai aff001; Akiyo Nakano aff002; Yuki Suzuki aff002; Naoki Kakuta aff002; Takashi Masui aff002; Hisakazu Yano aff002; Keiichi Mikasa aff001
Působiště autorů:
Center for Infectious Diseases, Nara Medical University, Kashihara, Japan
aff001; Department of Microbiology and Infectious Diseases, Nara Medical University, Kashihara, Japan
aff002
Vyšlo v časopise:
PLoS ONE 14(11)
Kategorie:
Research Article
doi:
https://doi.org/10.1371/journal.pone.0225210
Souhrn
Almost all cases of carbapenemase-producing Enterobacteriaceae infections in Japan are caused by blaIMP-positive Enterobacteriaceae (especially blaIMP-6) and infections caused by other types of carbapenemase-producing Enterobacteriaceae are quite rare. We examined drug resistance genes co-harboring with blaIMP-6 and their inoculum size effects. We screened β-lactamase genes, plasmid-mediated quinolone resistance (PMQR) genes, and aminoglycoside-modifying enzyme genes by PCR and performed sequencing for 14 blaIMP-6-positive Enterobacteriaceae. Further, all PMQR-positive isolates were submitted to conjugation and inoculum effect evaluation. Our data showed that 13 of the 14 isolates harbored CTX-M-2 and one co-harbored CTX-M-2 and CTX-M-1 as extended-spectrum β-lactamases. All isolates carried one or more PMQRs; aac(6’)-Ib-cr was the most prevalent (92.8%), and was followed by oqxA (64.3%), qnrS (50%), oqxAB (21.4%), and qnrB (14.3%). However, Klebsiella pneumoniae contains chromosomal OqxAB. Inoculum size effects were significant in all strains for meropenem, 13 strains for imipenem, 7 for levofloxacin, and 3 for amikacin. We observed that 11 of the experimental strains (100%), 8 strains (72.7%), and 1 strain showed inoculum size effects for meropenem, imipenem, and amikacin, respectively. However, four strains harbored qnr genes and two strains harbored qnr genes and QRDR mutations concurrently; no inoculum size effect was seen for levofloxacin. The blaIMP-6-positive Enterobacteriaceae that we studied was found to harbor at least one plasmid-mediated drug resistance gene. The inoculum size effect for carbapenems was thought to be mainly due to IMP-6-type metallo-β-lactamase; however qnrB and qnrS also had a minimal impact on the inoculum size effect for levofloxacin.
Klíčová slova:
Antibiotic resistance – Antibiotics – Antimicrobial resistance – Drug screening – Enterobacteriaceae – Polymerase chain reaction – Enterobacter infections
Zdroje
1. van Duin D, Kaye KS, Neuner EA, Bonomo RA. Carbapenem-resistant Enterobacteriaceae: a review of treatment and outcomes. Diagn Microbiol Infect Dis 2013;75: 115–120. doi: 10.1016/j.diagmicrobio.2012.11.009 23290507
2. Daikos GL, Tsaousi S, Tzouvelekis LS, Anyfantis I, Psichogiou M, Argyropoulou A, et al. Carbapenemase-producing Klebsiella pneumoniae bloodstream infections: lowering mortality by antibiotic combination schemes and the role of carbapenems. Antimicrob Agents Chemother 2014;58: 2322–2328. doi: 10.1128/AAC.02166-13 24514083
3. Morrill HJ, Pogue JM, Kaye KS, LaPlante KL. Treatment options for carbapenem-resistant Enterobacteriaceae infections. 2015. Open Forum Infect Dis 2:ofv050. doi: 10.1093/ofid/ofv050 26125030
4. Yano H, Ogawa M, Endo S, Kakuta R, Kanamori H, Inomata S, et al. High frequency of IMP-6 among clinical isolates of metallo-beta-lactamase-producing Escherichia coli in Japan. Antimicrob Agents Chemother 2012;56: 4554–4555. doi: 10.1128/AAC.00617-12 22664972
5. Yano H, Kuga A, Okamoto R, Kitasato H, Kobayashi T, Inoue M. Plasmid-encoded metallo-beta-lactamase (IMP-6) conferring resistance to carbapenems, especially meropenem. Antimicrob Agents Chemother 2001;45: 1343–1348. doi: 10.1128/AAC.45.5.1343-1348.2001 11302793
6. Kanazawa S, Sato T, Kohira N, Ito-Horiyama T, Tsuji M, Yamano Y. Susceptibility of imipenem-susceptible but meropenem-resistant BlaIMP-6 carrying Enterobacteriaceae to various antibacterials, including the siderophore cephalosporin cefiderocol. Antimicrob Agents Chemother 2017;66: e00576–e00517.
7. Pang F, Jia XQ, Zhao QG, Zhang Y. Factors associated to prevalence and treatment of carbapenem-resistant Enterobacteriaceae infections: a seven years retrospective study in three tertiary care hospitals. Ann Clin Microbiol Antimicrob 2018;17: 13. doi: 10.1186/s12941-018-0267-8 29571291
8. Robicsek A, Jacoby GA, Hooper DC. The worldwide emergence of plasmid-mediated quinolone resistance. Lancet Infect Dis 2006;6: 629–640. doi: 10.1016/S1473-3099(06)70599-0 17008172
9. Margaritis A, Galani I, Chatzikonstantinou M, Petrikkos G, Souli M. Plasmid-mediated quinolone resistance determinants among Gram-negative bacteraemia isolates: a hidden threat. J Med Microbiol 2017;66: 266–275. doi: 10.1099/jmm.0.000397 27902429
10. Yanat B Rodríguez-Martínez JM, Touati A. Plasmid-mediated quinolone resistance in Enterobacteriaceae: a systematic review with a focus on Mediterranean countries. Eur J Clin Microbiol Infect Dis 2017;36: 421–435. doi: 10.1007/s10096-016-2847-x 27889879
11. Wu N, Chen BY, Tian SF, Chu YZ. The inoculum effect of antibiotics against CTX-M-extended-spectrum beta-lactamase-producing Escherichia coli. Ann Clin Microbiol Antimicrob 2014;13: 45. doi: 10.1186/s12941-014-0045-1 25213463
12. Adler A, Ben-Dalak M, Chmelnitsky I, Carmeli Y. Effect of resistance mechanisms on the inoculum effect of carbapenem in Klebsiella pneumoniae isolates with borderline carbapenem resistance. Antimicrob Agents Chemother 2015;59: 5014–5017. doi: 10.1128/AAC.00533-15 25987630
13. Garoff L, Yadav K, Hughes D. Increased expression of Qnr is sufficient to confer clinical resistance to ciprofloxacin in Escherichia coli. J Antimicrob Chemother 2018:73: 348–352. doi: 10.1093/jac/dkx375 29106520
14. Nakano A, Nakano R, Suzuki Y, Saito K, Kasahara K, Endo S, et al. Rapid identification of bla(IMP-1) and bla(IMP-6) by multiplex amplification refractory mutation system PCR. Ann Lab Med 2018;38: 378–380. doi: 10.3343/alm.2018.38.4.378 29611391
15. Poirel L, Walsh TR, Cuvillier V, Nordmann P. Multiplex PCR for detection of acquired carbapenemase genes. Diagn Microbiol Infect 2011;70: 119–123.
16. Shibata N, Kurokawa H, Doi Y, Yagi T, Yamane K, Wachino J, et al. PCR classification of CTX-M-type beta-lactamase genes identified in clinically isolated Gram-negative bacilli in Japan. Antimicrob Agents Chemother 2006;50: 791–795. doi: 10.1128/AAC.50.2.791-795.2006 16436748
17. Guillard T, Moret H, Brasme L, Carlier A, Vernet-Garnier V, Cambau E, et al. Rapid detection of qnr and qepA plasmid-mediated quinolone resistance genes using real-time PCR. Diagn Microbiol Infect Dis 2011;70: 253–259. doi: 10.1016/j.diagmicrobio.2011.01.004 21596225
18. Casin I, Hanau-Bercot B, Podglajen I, Vahaboglu H, Collatz E. Salmonella enterica serovar Typhimurium blaPER-1-carrying plasmid pSTI1 encodes an extended-spectrum aminoglycoside 6'-N-acetyltransferase of Type Ib. Antimicrob Agents Chemother 2003;47: 697–703. doi: 10.1128/AAC.47.2.697-703.2003 12543680
19. Park CH, Robicsek A. Jacoby GA, Sahm D, Hooper DC. Prevalence in the United States of aac(6')-Ib-cr encoding a ciprofloxacin-modifying enzyme. Antimicrob Agents Chemother 2006;50: 3953–3955. doi: 10.1128/AAC.00915-06 16954321
20. Kim HB, Wang M, Park CH, Kim EC, Jacoby GA, Hooper DC. oqxAB encoding a multidrug efflux pump in human clinical isolates of Enterobacteriaceae. Antimicrob Agents Chemother 2009;53: 3582–3584. doi: 10.1128/AAC.01574-08 19528276
21. Mingeot-Leclercq MP, Glupczynski Y, Tulkens PM. Aminoglycosides: activity and resistance. Antimicrob Agents Chemother 1999;43: 727–737. 10103173
22. Ramirez MS, Tolmasky ME. Aminoglycoside modifying enzymes. Drug Resist Update 2010;13: 151–171.
23. Galani I, Souli M, Mitchell N, Chryssouli Z, Giamarellou H. Presence of plasmid-mediated quinolone resistance in Klebsiella pneumoniae and Escherichia coli isolates possessing blaVIM-1 in Greece. Int J Antimicrob Agents 2010;36: 252–254. doi: 10.1016/j.ijantimicag.2010.05.004 20580536
24. Nakano R, Okamoto R, Nakano A, Nagano N, Abe M, Tansho-Nagakawa S, et al. Rapid assay for detecting gyrA and parC mutations associated with fluoroquinolone resistance in Enterobacteriaceae. J Microbiol Methods 2013;94: 213–216. doi: 10.1016/j.mimet.2013.06.019 23816531
25. Qiang YZ, Qin T, Fu W, Cheng WP, Li YS, Yi G. Use of a rapid mismatch PCR method to detect qyrA and parC mutations in ciprofloxacin-resistant clinical isolates of Escherichia coli. J Antimicrob Chemother 2002;49: 549–552. doi: 10.1093/jac/49.3.549 11864958
26. Clinical and Laboratory Standards Institute. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard- Tenth Edition. Document M07-A10; Wayne, PU.
27. Clinical and Laboratory Standards Institute: Performance standards for antimicrobial susceptibility testing; Twenty-Fifth informational supplement. Document M100-S250; Wayne, PU.
28. EUCAST. Breakpoint tables for interpretation of MICs and zone diameters version 8.1. http://www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files/Breakpoint_tables/v_8.1_Breakpoint_Tables.pdf.
29. Rice LB. Mechanisms of resistance and clinical relevance of resistance to β-lactams, glycopeptides, and fluoroquinolones. Mayo Clinic Proc. 2012;87: 198–208.
30. Qureshi ZA, Paterson DL, Potoski BA, Kilayko MC, Sandovsky G, Sordillo E, et al. Treatment outcome of bacteremia due to KPC-producing Klebsiella pneumoniae: Superiority of combination antimicrobial regimens. Antimicrob Agents Chemother 2013;56: 2108–2113.
31. Weisenberg SA, Morgan DJ, Espinal-Witter R, Larone DH. Clinical outcomes of patients with Klebsiella pneumoniae carbapenemase-producing K. pneumoniae after treatment with imipenem or meropenem. Diagn Microbiol Infect Dis 2009;64: 233–235. doi: 10.1016/j.diagmicrobio.2009.02.004 19345034
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