Transcriptional analysis of amino acid, metal ion, vitamin and carbohydrate uptake in butanol-producing Clostridium beijerinckii NRRL B-598


Autoři: Maryna Vasylkivska aff001;  Katerina Jureckova aff002;  Barbora Branska aff001;  Karel Sedlar aff002;  Jan Kolek aff001;  Ivo Provaznik aff002;  Petra Patakova aff001
Působiště autorů: Department of Biotechnology, University of Chemistry and Technology Prague, Prague, Czech Republic aff001;  Department of Biomedical Engineering, Brno University of Technology, Brno, Czech Republic aff002
Vyšlo v časopise: PLoS ONE 14(11)
Kategorie: Research Article
doi: 10.1371/journal.pone.0224560

Souhrn

In-depth knowledge of cell metabolism and nutrient uptake mechanisms can lead to the development of a tool for improving acetone-butanol-ethanol (ABE) fermentation performance and help to overcome bottlenecks in the process, such as the high cost of substrates and low production rates. Over 300 genes potentially encoding transport of amino acids, metal ions, vitamins and carbohydrates were identified in the genome of the butanol-producing strain Clostridium beijerinckii NRRL B-598, based on similarity searches in protein function databases. Transcriptomic data of the genes were obtained during ABE fermentation by RNA-Seq experiments and covered acidogenesis, solventogenesis and sporulation. The physiological roles of the selected 81 actively expressed transport genes were established on the basis of their expression profiles at particular stages of ABE fermentation. This article describes how genes encoding the uptake of glucose, iron, riboflavin, glutamine, methionine and other nutrients take part in growth, production and stress responses of C. beijerinckii NRRL B-598. These data increase our knowledge of transport mechanisms in solventogenic Clostridium and may be used in the selection of individual genes for further research.

Klíčová slova:

Carbohydrates – Clostridium – Fermentation – Glucose – Vitamins – Zinc – Butanol – Riboflavin


Zdroje

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