Effective methods for the inactivation of Francisella tularensis

Autoři: Mika Azaki aff001;  Akihiko Uda aff001;  Deyu Tian aff003;  Katsuyoshi Nakazato aff002;  Akitoyo Hotta aff001;  Yasuhiro Kawai aff004;  Keita Ishijima aff001;  Yudai Kuroda aff001;  Ken Maeda aff001;  Shigeru Morikawa aff001
Působiště autorů: Department of Veterinary Science, National Institute of Infectious Diseases, Tokyo Japan aff001;  Department of Correlative Study in Physics and Chemistry, Graduate School of Integrated Basic Sciences, Nihon University, Tokyo, Japan aff002;  CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China aff003;  Division of Biosafety Control and Research, National Institute of Infectious Diseases, Tokyo, Japan aff004;  Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan aff005
Vyšlo v časopise: PLoS ONE 14(11)
Kategorie: Research Article
doi: 10.1371/journal.pone.0225177


Francisella tularensis (F. tularensis) is highly pathogenic to humans and must be handled under biosafety level 3 conditions. Samples used for the diagnosis and experimental analysis must be completely inactivated, although methods for the inactivation of F. tularensis are limited. In this study, effective methods for the inactivation of F. tularensis SCHU P9 and five other strains were determined by comparisons of colony-forming units between treated and control samples. The results showed that F. tularensis SCHU P9 was denatured by heat treatment (94°C for 3 min and 56°C for 30 min), filtration with a 0.22 μm filter, and the use of various solutions (i.e. >70% ethanol, methanol, acetone, and 4% paraformaldehyde). F. tularensis SCHU P9 remained viable after treatment with 50% ethanol for 1 min, filtration with a 0.45 μm filter, and treatments with detergents (i.e. 1% lithium dodecyl sulfate buffer, 1% Triton X-100 and 1% Nonidet P-40) at 4°C for 24 h. Additionally, F. tularensis SCHU P9 suspended in fetal bovine serum in plastic tubes was highly resistant to ultraviolet radiation compared to suspensions in water and chemically defined medium. The methods for inactivation of F. tularensis SCHU P9 was applicable to the other five strains of F. tularensis. The data presented in this study could be useful for the establishment of guidelines and standard operating procedures (SOP) to inactivate the contaminated samples in not only F. tularensis but also other bacteria.

Klíčová slova:

Acetones – Detergents – Ethanol – Francisella tularensis – Government laboratories – Heat treatment – Ultraviolet radiation – Filter sterilization


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