Importance of thorough tissue and cellular level characterization of targeted drugs in the evaluation of pharmacodynamic effects


Autoři: Dustin K. Bauknight aff001;  Victoria Osinski aff003;  Siva Sai Krishna Dasa aff001;  Anh T. Nguyen aff003;  Melissa A. Marshall aff003;  Julia Hartman aff001;  Matthew Harms aff005;  Gavin O’Mahony aff005;  Jeremie Boucher aff005;  Alexander L. Klibanov aff001;  Coleen A. McNamara aff003;  Kimberly A. Kelly aff001
Působiště autorů: Department of Biomedical Engineering, University of Virginia, Charlottesville, VA, United States of America aff001;  Cancer Center, University of Virginia, Charlottesville, VA, United States of America aff002;  Robert M. Berne Cardiovascular Research Center, University of Virginia, Charlottesville, VA, United States of America aff003;  Department of Pathology, University of Virginia, Charlottesville, VA, United States of America aff004;  Research and Early Development, Cardiovascular, Renal and Metabolism (CVRM), BioPharmaceuticals R&D, AstraZeneca, Gothenburg, Sweden aff005;  The Lundberg Laboratory for Diabetes Research, University of Gothenburg, Gothenburg, Sweden aff006;  Wallenberg Centre for Molecular and Translational Medicine, University of Gothenburg, Gothenburg, Sweden aff007;  Department of Medicine, Division of Cardiovascular Medicine, University of Virginia, Charlottesville, VA, United States of America aff008
Vyšlo v časopise: PLoS ONE 14(11)
Kategorie: Research Article
doi: 10.1371/journal.pone.0224917

Souhrn

Targeted nanoparticle delivery is a promising strategy for increasing efficacy and limiting side effects of therapeutics. When designing a targeted liposomal formulation, the in vivo biodistribution of the particles must be characterized to determine the value of the targeting approach. Peroxisome proliferator-activated receptor (PPAR) agonists effectively treat metabolic syndrome by decreasing dyslipidemia and insulin resistance but side effects have limited their use, making them a class of compounds that could benefit from targeted liposomal delivery. The adipose targeting sequence peptide (ATS) could fit this role, as it has been shown to bind to adipose tissue endothelium and induce weight loss when delivered conjugated to a pro-apoptotic peptide. To date, however, a full assessment of ATS in vivo biodistribution has not been reported, leaving important unanswered questions regarding the exact mechanisms whereby ATS targeting enhances therapeutic efficacy. We designed this study to evaluate the biodistribution of ATS-conjugated liposomes loaded with the PPARα/γ dual agonist tesaglitazar in leptin-deficient ob/ob mice. The ATS-liposome biodistribution in adipose tissue and other organs was examined at the cellular and tissue level using microscopy, flow cytometry, and fluorescent molecular tomography. Changes in metabolic parameters and gene expression were measured by target and off-target tissue responses to the treatment. Unexpectedly, ATS targeting did not increase liposomal uptake in adipose relative to other tissues, but did increase uptake in the kidneys. Targeting also did not significantly alter metabolic parameters. Analysis of the liposome cellular distribution in the stromal vascular fraction with flow cytometry revealed high uptake by multiple cell types. Our findings highlight the need for thorough study of in vivo biodistribution when evaluating a targeted therapy.

Klíčová slova:

Adipose tissue – Drug therapy – Flow cytometry – Insulin – Kidneys – Liposomes – Macrophages – Nanoparticles


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