Metabolomic response of Euglena gracilis and its bleached mutant strain to light


Autoři: Qing Shao aff001;  Lang Hu aff002;  Huan Qin aff001;  Yerong Liu aff001;  Xing Tang aff001;  Anping Lei aff001;  Jiangxin Wang aff001
Působiště autorů: Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen Uni aff001;  Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen Uni aff001;  College of Life Sciences and Technology, Hubei Engineering University, Xiaogan, China aff002
Vyšlo v časopise: PLoS ONE 14(11)
Kategorie: Research Article
doi: 10.1371/journal.pone.0224926

Souhrn

Euglena, a new superfood on the market, is a nutrient-rich, green single-celled microorganism that features the characteristics of both plant and animal. When cultivated under different conditions, Euglena produces different bioactive nutrients. Interestingly, Euglena is the only known microorganism whose chloroplasts are easy to lose under stress and become permanently bleached. We applied gas chromatography-mass spectrometry (GC-MS) to determine the metabolomes of wild-type (WT) Euglena gracilis and its bleached mutant OflB2 under light stimulation. We found a significant metabolic difference between WT and OflB2 cells in response to light. An increase of membrane components (phospholipids and acylamides) was observed in WT, while a decrease of some stimulant metabolites was detected in OflB2. These metabolomic changes after light stimulation are of great significance to the development of Euglena chloroplasts and their communications with the nucleus.

Klíčová slova:

Drug metabolism – Gas chromatography-mass spectrometry – Chloroplasts – Light – Metabolites – Metabolomics – Protein metabolism – Membrane metabolism


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2019 Číslo 11