Application of CPI cutoff value based on parentage testing of duos and trios typed by four autosomal kits


Autoři: Hongmei Gao aff001;  Chang Wang aff001;  Ruxia Zhang aff003;  Hanyang Wu aff004;  Shanhui Sun aff001;  Dongjie Xiao aff001;  Yunshan Wang aff001;  Maoxiu Zhang aff001
Působiště autorů: Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong, China aff001;  Jinan Di’en Forensic Institute of Jinan Central Hospital, Jinan, Shandong, China aff002;  Department of Nursing, Shandong Medical College, Jinan, Shandong, China aff003;  Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China aff004
Vyšlo v časopise: PLoS ONE 14(11)
Kategorie: Research Article
doi: 10.1371/journal.pone.0225174

Souhrn

In this study, we analyzed the application of four autosomal kits and the sensitivity of the combined paternity index (CPI) cutoff value (CPI≥10000) in parentage testing. First, 1442 real trios and 803 real duos were tested using the Goldeneye 25A kit. The Goldeneye 25A kit covers the autosomal short tandem repeat (STR) loci of the other three kits, so we calculated the CPI value of every case for the four kits. Second, three complex close relative kinship cases were also analyzed to evaluate the application of the CPI cutoff value. The CPI values of all trio cases were higher than 10000 using the four kits; the CPI values of all duo cases were higher than 10000 using the Goldeneye 25A kit; and the CPI values of a portion of the duo cases were lower than 10000 using the other three kits. In the three complex close relative cases, the alleged father or mother was not excluded using 40 autosomal STRs. Adding X chromosome short tandem repeats (X-STR) and samples of biological fathers or mothers, the conclusions were confirmed. The four kits were adequate to draw conclusions in the trio cases; the Goldeneye 25A Kit was adequate to draw conclusions in the duo cases; and the other three kits were not sufficient for a portion of the duo cases. The CPI cutoff value was sensitive for real trio and duo cases. In complex close relative kinship cases, high CPI values may result in false conclusions.

Klíčová slova:

DNA extraction – Forensics – Genetic loci – China – X chromosomes – Y chromosomes – Tandem repeats – Pentas


Zdroje

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Článek vyšel v časopise

PLOS One


2019 Číslo 11