Arabidopsis TRM5 encodes a nuclear-localised bifunctional tRNA guanine and inosine-N1-methyltransferase that is important for growth

Autoři: Qianqian Guo aff001;  Pei Qin Ng aff002;  Shanshan Shi aff001;  Diwen Fan aff001;  Jun Li aff002;  Jing Zhao aff002;  Hua Wang aff003;  Rakesh David aff004;  Parul Mittal aff005;  Trung Do aff002;  Ralph Bock aff006;  Ming Zhao aff001;  Wenbin Zhou aff001;  Iain Searle aff002
Působiště autorů: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China aff001;  School of Biological Sciences, School of Agriculture, Food and Wine, The University of Adelaide and Shanghai Jiao Tong University Joint International Centre for Agriculture and Health, The University of Adelaide, Adelaide, Adelaide, Australia aff002;  National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China aff003;  ARC Centre of Excellence in Plant Energy Biology, School of Agriculture, Food and Wine, University of Adelaide, Adelaide, Australia aff004;  Adelaide Proteomics Centre, School of Biological Sciences, The University of Adelaide, SA, Australia aff005;  Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg, Potsdam-Golm, Germany aff006
Vyšlo v časopise: PLoS ONE 14(11)
Kategorie: Research Article
doi: 10.1371/journal.pone.0225064


Modified nucleosides in tRNAs are critical for protein translation. N1-methylguanosine-37 and N1-methylinosine-37 in tRNAs, both located at the 3’-adjacent to the anticodon, are formed by Trm5. Here we describe Arabidopsis thaliana AtTRM5 (At3g56120) as a Trm5 ortholog. Attrm5 mutant plants have overall slower growth as observed by slower leaf initiation rate, delayed flowering and reduced primary root length. In Attrm5 mutants, mRNAs of flowering time genes are less abundant and correlated with delayed flowering. We show that AtTRM5 complements the yeast trm5 mutant, and in vitro methylates tRNA guanosine-37 to produce N1-methylguanosine (m1G). We also show in vitro that AtTRM5 methylates tRNA inosine-37 to produce N1-methylinosine (m1I) and in Attrm5 mutant plants, we show a reduction of both N1-methylguanosine and N1-methylinosine. We also show that AtTRM5 is localized to the nucleus in plant cells. Proteomics data showed that photosynthetic protein abundance is affected in Attrm5 mutant plants. Finally, we show tRNA-Ala aminoacylation is not affected in Attrm5 mutants. However the abundance of tRNA-Ala and tRNA-Asp 5’ half cleavage products are deduced. Our findings highlight the bifunctionality of AtTRM5 and the importance of the post-transcriptional tRNA modifications m1G and m1I at tRNA position 37 in general plant growth and development.

Klíčová slova:

Arabidopsis thaliana – Flowering plants – Nucleosides – Protein translation – Saccharomyces cerevisiae – Seedlings – Transfer RNA – Yeast


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