Multiple innate antibacterial immune defense elements are correlated in diverse ungulate species


Autoři: Brian S. Dugovich aff001;  Lucie L. Crane aff002;  Benji B. Alcantar aff002;  Brianna R. Beechler aff002;  Brian P. Dolan aff002;  Anna E. Jolles aff002
Působiště autorů: Department of Integrative Biology, Oregon State University, Corvallis, OR, United States of America aff001;  Department of Biomedical Sciences, Oregon State University, Corvallis, OR, United States of America aff002;  Wildlife Safari, Winston, OR, United States of America aff003
Vyšlo v časopise: PLoS ONE 14(11)
Kategorie: Research Article
doi: 10.1371/journal.pone.0225579

Souhrn

In this study, we aimed to evaluate to what extent different assays of innate immunity reveal similar patterns of variation across ungulate species. We compared several measures of innate antibacterial immune function across seven different ungulate species using blood samples obtained from captive animals maintained in a zoological park. We measured mRNA expression of two receptors involved in innate pathogen detection, toll-like receptors 2 and 5 (TLR2 and 5), the bactericidal capacity of plasma, as well as the number of neutrophils and lymphocytes. Species examined included aoudad (Ammotragus lervia), American bison (Bison bison bison), yak (Bos grunniens), Roosevelt elk (Cervus canadensis roosevelti), fallow deer (Dama dama), sika deer (Cervus nippon), and Damara zebra (Equus quagga burchellii). Innate immunity varied among ungulate species. However, we detected strong, positive correlations between the different measures of innate immunity–specifically, TLR2 and TLR5 were correlated, and the neutrophil to lymphocyte ratio was positively associated with TLR2, TLR5, and bacterial killing ability. Our results suggest that ecoimmunological study results may be quite robust to the choice of assays, at least for antibacterial innate immunity; and that, despite the complexity of the immune system, important sources of variation in immunity in natural populations may be discoverable with comparatively simple tools.

Klíčová slova:

Antibacterials – Blood plasma – Immune response – Lymphocytes – Neutrophils – Polymerase chain reaction – Toll-like receptors – White blood cells


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Článek vyšel v časopise

PLOS One


2019 Číslo 11