Structural characterization of scorpion peptides and their bactericidal activity against clinical isolates of multidrug-resistant bacteria


Autoři: Catherine Cesa-Luna aff001;  Jesús Muñoz-Rojas aff001;  Gloria Saab-Rincon aff002;  Antonino Baez aff001;  Yolanda Elizabeth Morales-García aff001;  Víctor Rivelino Juárez-González aff004;  Verónica Quintero-Hernández aff001
Působiště autorů: Ecology and Survival of Microorganisms Group (ESMG), Laboratorio de Ecología Molecular Microbiana (LEMM), Centro de Investigaciones en Ciencias Microbiológicas (CICM), Instituto de Ciencias (IC), Benemérita Universidad Autónoma de Puebla (BUAP), Puebla, P aff001;  Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México aff002;  Licenciatura en Biotecnología, Facultad de Ciencias Biológicas, BUAP, Puebla, Puebla, México aff003;  Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Mor., México aff004;  CONACYT-ESMG, LEMM, CICM, IC, BUAP, Puebla, Puebla, México aff005
Vyšlo v časopise: PLoS ONE 14(11)
Kategorie: Research Article
doi: 10.1371/journal.pone.0222438

Souhrn

Scorpion venom peptides represent a novel source of antimicrobial peptides (AMPs) with broad-spectrum activity. In this study, we determined the minimum bactericidal concentration (MBC) of three scorpion AMPs, Uy234, Uy17, and Uy192, which are found in the venomous glands of the Urodacus yaschenkoi scorpion, against the clinical isolates of multidrug-resistant (MDR) bacteria. In addition, we tested the activity of a consensus AMP designed in our laboratory based on some previously reported IsCT-type (cytotoxic linear peptide) AMPs with the aim of obtaining higher antimicrobial activity. All peptides tested showed high antimicrobial activity against MDR clinical isolates, with the highest activity against β-hemolytic Streptococcus strains. The hemolytic activity was determined against human red blood cells and was significantly lower than that of previously reported AMPs. The α-helical structure of the four AMPs was confirmed by circular dichroism (CD). These results suggest that the four peptides can be valuable tools for the design and development of AMPs for use in the inhibition of MDR pathogenic bacteria. A clear index of synergism and additivity was found for the combination of QnCs-BUAP + Uy234, which makes these peptides the most promising candidates against pathogenic bacteria.

Klíčová slova:

Antimicrobials – Cell membranes – Gram negative bacteria – Pneumococcus – Streptococcus – Venoms – Scorpions – Burkholderia cepacia complex


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