Diagnostic plasma miRNA-profiles for ovarian cancer in patients with pelvic mass

Autoři: Douglas Nogueira Perez Oliveira aff001;  Anting Liu Carlsen aff002;  Niels H. H. Heegaard aff002;  Kira Philipsen Prahm aff001;  Ib Jarle Christensen aff001;  Claus K. Høgdall aff004;  Estrid V. Høgdall aff001
Působiště autorů: Department of Pathology, Herlev Hospital, University of Copenhagen, Herlev, Denmark aff001;  Department of Autoimmunology and Biomarkers, Statens Serum Institut, Copenhagen, Denmark aff002;  Department of Congenital Disorders, Statens Serum Institut, Copenhagen, Denmark aff003;  Department of Gynaecology, Juliane Marie Centre, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark aff004
Vyšlo v časopise: PLoS ONE 14(11)
Kategorie: Research Article
doi: 10.1371/journal.pone.0225249



Ovarian cancer is the fifth most common cancer in women worldwide. Moreover, there are no reliable minimal invasive tests to secure the diagnosis of malignant pelvic masses. Cell-free, circulating microRNAs have the potential as diagnostic biomarkers in cancer. Here, we performed and validated a miRNA panel with the potential to distinguish OC from benign pelvic masses.


The profile of plasma microRNA was determined with a panel of 46 candidates in a discovery group and a validation group, each consisting of 190 pre-surgery plasma samples from age-matched patients with malignant (n = 95) and benign pelvic mass (n = 95), by real time RT-qPCR.


Four up-regulated (miR-200c-3p, miR-221-3p, miR-21-5p, and miR-484) and two down-regulated (miR-195-5p and miR-451a) microRNAs were discovered. From those, miR-200c-3p and miR-221-3p were further confirmed in a validation cohort. A combination of these 2 microRNAs together with CA-125 yielded an overall diagnostic accuracy of AUC = 0.96.


We showed consistent plasma microRNA profiles that provide independent diagnostic information of late stage OC.

Klíčová slova:

Adenocarcinomas – Biomarkers – Cancer detection and diagnosis – Diagnostic medicine – MicroRNAs – Ovarian cancer – Surgical and invasive medical procedures – Surgical oncology


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