Fibre and extracellular matrix contributions to passive forces in human skeletal muscles: An experimental based constitutive law for numerical modelling of the passive element in the classical Hill-type three element model


Autoři: Lorenzo Marcucci aff001;  Michela Bondì aff001;  Giulia Randazzo aff001;  Carlo Reggiani aff001;  Arturo N. Natali aff002;  Piero G. Pavan aff002
Působiště autorů: Department of Biomedical Sciences, University of Padova, Padova, Italy aff001;  Centre for Mechanics of Biological Materials, University of Padova, Padova, Italy aff002;  Kinesiology Research Center, Garibaldijeva, Koper, Slovenia aff003;  Department of Industrial Engineering, University of Padova, Padova, Italy aff004;  Fondazione Istituto di Ricerca Pediatrica Città della Speranza, Corso Stati Uniti 4, Padova, Italy aff005
Vyšlo v časopise: PLoS ONE 14(11)
Kategorie: Research Article
doi: 10.1371/journal.pone.0224232

Souhrn

The forces that allow body movement can be divided into active (generated by sarcomeric contractile proteins) and passive (sustained by intra-sarcomeric proteins, fibre cytoskeleton and extracellular matrix (ECM)). These are needed to transmit the active forces to the tendon and the skeleton. However, the relative contribution of the intra- and extra- sarcomeric components in transmitting the passive forces is still under debate. There is limited data in the literature about human muscle and so it is difficult to make predictions using multiscale models, imposing a purely phenomenological description for passive forces. In this paper, we apply a method for the experimental characterization of the passive properties of fibres and ECM to human biopsy and propose their clear separation in a Finite Element Model. Experimental data were collected on human single muscle fibres and bundles, taken from vastus lateralis muscle of elderly subjects. Both were progressively elongated to obtain two stress-strain curves which were fitted to exponential equations. The mechanical properties of the extracellular passive components in a bundle of fibres were deduced by the subtraction of the passive tension observed in single fibres from the passive tension observed in the bundle itself. Our results showed that modulus and tensile load bearing capability of ECM are higher than those of fibres and defined their quantitative characterization that can be used in macroscopic models to study their role in the transmission of forces in physiological and pathophysiological conditions.

Klíčová slova:

Behavior – Extracellular matrix – Muscle components – Muscle fibers – Muscle proteins – Skeletal muscles – Tangents


Zdroje

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