Monitoring fine root growth to identify optimal fertilization timing in a forest plantation: A case study in Northeast Vietnam

Autoři: Tran Van Do aff001;  Nguyen Toan Thang aff001;  Vu Tien Lam aff001;  Dang Van Thuyet aff001;  Phung Dinh Trung aff001;  Tran Hoang Quy aff001;  Nguyen Thi Thu Phuong aff001;  Ly Thi Thanh Huyen aff001;  Nguyen Huu Thinh aff001;  Nguyen Van Tuan aff001;  Dao Trung Duc aff001;  Dang Thi Hai Ha aff001;  Duong Quang Trung aff001;  Ho Trung Luong aff001;  Nguyen Thi Hoai Anh aff001;  Patrick Nykiel aff002
Působiště autorů: Silviculture Research Institute, Vietnamese Academy of Forest Sciences, Hanoi, Vietnam aff001;  Independent Australian Researcher, Vietnamese Academy of Forest Sciences, Hanoi, Vietnam aff002
Vyšlo v časopise: PLoS ONE 14(11)
Kategorie: Research Article
doi: 10.1371/journal.pone.0225567


Fertilizer is applied widely to improve the productivity of plantations. Traditionally, fertilization is conducted in spring and/or in the early rainy season, and it is believed to support the growth of planted trees in the growing season. Little attention to date has been paid on identification of the optimal timing of fertilization and fertilizer dose. In this study, application of the fine root monitoring technique in identifying optimal fertilization timing for an Acacia plantation in Vietnam is described. The study used two fertilizer doses (100 and 200 g NPK/tree) and three fertilization timings (in spring; in the early rainy season; and based on the fine root monitoring technique to identify when the fine roots reach their growth peak). As expected fertilization timings significantly affected growth and above-ground biomass (AGB) of the plantation. Fertilization based on the fine root monitoring technique resulted in the highest growths and AGB, followed by fertilization in the early rainy season and then in spring. Applying fertilizer at 200 g NPK/tree based on the fine root monitoring technique increased diameter at breast height (DBH) by 16%, stem height by 8%, crown diameter (Dc) by 16%, and AGB by 40% as compared to early rainy season fertilization. Increases of 32% DBH, 23% stem height, 44% Dc, and 87% AGB were found in fertilization based on fine root monitoring technique compared to spring fertilization. This study concluded that forest growers should use the fine root monitoring technique to identify optimal fertilization timing for higher productivity.

Klíčová slova:

Fertilizers – Fine roots – Forests – Root growth – Seasons – Spring – Trees – Acacia


1. Indufor. A12-06869 Strategic review on the future of forest plantation (ID 11914). Forest Stewardship Council, Helsinki Finland; 2012.

2. FAO. How are the world’s forests changing? Global Forest Resources Assessment 2015. Food and Agriculture Organization of the United Nations 2016; pp. 54.

3. Lee K.L., Ong K.H., King P.J.H., Chubo J.K. and Su D.S.A. Stand productivity, carbon content, and soil nutrients in different stand ages of Acacia auriculiformis in Sarawak, Malaysia. Turkish Journal of Agriculture and Forestry 2015; 39:154–161.

4. Bouma J. Sustainable land use as a future focus for pedology. Soil Science Society of America Journal 1994; 58:645–646.

5. Sophie W., Leigh A.K.K. and Grayston S.J. Effects of long-term fertilization of forest soils on potential nitrification and on the abundance and community structure of ammonia oxidizers and nitrite oxidizers. FEMS Microbiology and Ecology 2011; 79:142–154.

6. Zech W. and Drechsel P. Degradation and amelioration of soils and tree nutrient status without and with mineral fertilizer. In: Schulte A. and Ruhiyat D. (eds.) Soils of tropical forest ecosystems 5, 5–30. Mulawarman University Press, Samarinda, Indonesia; 1995.

7. Hung T.T., Almeida A.C., Eyles A., Ratkowsky D., Lam V.T. and Mohammed C. Maximizing growth and sawlog production from Acacia hybrid plantations in Vietnam. New Forests, in press; 2009.

8. Shi W., Grossnickle S.C., Shuchai G.L. and Liu S.U. Fertilization and irrigation regimes influence on seedling attributes and field performance of Pinus tabuliformis Carr. Forestry 2019; 92:97–107.

9. Fox T.R., Allen H.L., Albaugh T.J., Rubilar R. and Carlson C.A. Tree nutrition and forest fertilization of pine plantations in the Southern United States. Southern Journal of Applied Forestry 2007; 31:5–11.

10. Doran J.W. Soil health and global sustainability: translating science into practice. Agriculture, Ecosystems & Environment 2002; 88:119–127.

11. Bich N.V., Eyles A., Mendham D., Dong T.L., Ratkowsky D., Evans K.J., et al. Contribution of harvest residues to nutrient cycling in a tropical Acacia mangium Willd. plantation. Forests 2018: 9:577.

12. Sulaiman A., Kadir Q.R.A. and Midon M.S. Effects of fertilizer on wood properties of plantation crown Acacia mangium. Journal of Tropical Forest Science 1990; 4:119–126.

13. Goncalves J.L.M., Wichert M.C.P., Gava J.L., Masetto A.V., Arthur J.C.J, Serrano, et al. Soil fertility and growth of Eucalyptus grandis in Brazil under different residue management practices. Southern Hemisphere Forestry Journal 2007; 69:95–102.

14. Guo J., Wu Y., Wang B., Lu Y., Cao F. and Wang G. The effects of fertilization on the growth and physiological characteristics of Ginkgo biloba L. Forests 2016; 7:293.

15. Forrester D.I. Growth responses to thinning, pruning and fertilizer application in Eucalyptus plantations: A review of their production ecology and Interactions. Forest Ecology and Management 2013; 310:336–347.

16. Lamani V.K., Patil S.K. and Manjunath G.O. Growth of Acacia auriculiformis as influenced by N, P and K fertilizers. Karnataka Journal of Agricultural Science 2004; 17:872–874.

17. Forrester D.I., Collopy J.J., Beadle C.L., Warren C.R. and Baker T.G. Effect of thinning, pruning and nitrogen fertilizer application on transpiration, photosynthesis and water-use efficiency in a young Eucalyptus nitens plantation. Forest Ecology and Management 2012; 266:286–300.

18. Silva P.H.M. Poggiani F. Libardi P.L. and Gonçalves A.N. Fertilizer management of eucalypt plantations on sandy soil in Brazil: Initial growth and nutrient cycling. Forest Ecology and Management 2013; 301:67–78.

19. Timander P. Fertilization in Eucalyptus urophylla plantations in Guangxi, southern China. Master thesis, Southern Swedish Forest Research Centre, Swedish University of Agricultural Sciences; 2011.

20. Pham T.D., Ngo V.N. and Nguyen T.B. Research on silvicultural practices for growing Acacia hybrid in Binh Phuoc Province to supply pulp wood. Scientific Report, Forest Science Institute of Vietnam. Hanoi, Vietnam; 2005.

21. Dickens E.D., Moorhead D.J. and McElvany B. Pine plantation fertilization. Better Crops 2003; 87:12–15.

22. R. Fox T., Allen H.L., Albaugh T.J., Rubilar R. and Carlson C.A. Tree nutrition and forest fertilization of pine plantations in the Southern United States. Southern Journal of Applied Forestry 2007; 31:5–11.

23. Blazier M.A., Scott D.A. and Coleman R. Mid-rotation silviculture timing influences nitrogen mineralization of loblolly pine plantations in the mid-south USA. Forests 2015; 6:1061–1082.

24. Timander P. Fertilization in Eucalyptus urophylla plantations in southern China. Master Thesis. Swedish University of Agricultural Sciences 2011.

25. Du X. and Wei X. Definition of fine roots on the basis of the root anatomy, diameter, and branch orders of one-year old Fraxinus mandshurica seedlings. Journal of Forestry Research 2017; 29:1321–1327.

26. Helmisaari H.S., Lehto T. and Makkonen K. Fine roots and soil properties. In: Mälkönen E. (eds) Forest Condition in a Changing Environment. Forestry Sciences, vol 65. Springer, Dordrecht; 2000.

27. Tran V.D., Tamotsu S. and Kozan O. A new approach for estimating fine root production in forests: A combination of ingrowth core and scanner. Trees 2016; 30:545–554.

28. Osawa A. and Aizawa R. A new approach to estimate fine root production, mortality, and decomposition using litter bag experiments and soil core techniques. Plant and Soil 2012; 355:167–181.

29. Tran V.D., Osawa A. and Sato T. Estimation of fine-root production using rates of diameter-dependent root mortality, decomposition and thickening in forests. Tree Physiology 2016; 36:513–523. doi: 10.1093/treephys/tpv121 26614784

30. QCVN02. Vietnam Building Code Natural Physical & Climatic Data for Construction. Hanoi 2009; 324p.

31. Dang V.T. Research on silviculture techniques for intensive plantation toward timber supply. Scientific report. Vietnamese Academy of Forest Sciences. Hanoi, Vietnam; 2010.

32. Mainwaring D.B., Maguire D.A. and Perakis S.S. Three-year growth response of young Douglas-fir to nitrogen, calcium, phosphorus, and blended fertilizers in Oregon and Washington. Forest Ecology and Management 2014; 327:178–188.

33. Ferguson J.C. and Smucker A.J.M. Modifications of the minirhizotron video camera system for measuring spatial and temporal root dynamics. Soil Science Society of America Journal 1989; 53:1601–1605.

34. Dannoura M., Kominami Y., Oguma H. and Kanazawa Y. The development of an optical scanner method for observation of plant root dynamics. Plant Root 2008; 2:14–18.

35. Di Iorio A., Montagnoli A., Terzaghi M., Scippa G.S. and Chiatante D. Effect of tree density on root distribution in Fagus sylvatica stands: a semi-automatic digitising device approach to trench wall method. Trees 2013; 27:1503–1513.

36. Cislaghi A., Bordoni M., Meisina C. and Bischetti G.B. Soil reinforcement provided by the root system of grapevines: Quantification and spatial variability. Ecological Engineering 2017; 109: 169–185.

37. Vergani C., Werlen M., Conedera M., Cohen D. and Schwarz M. Investigation of root reinforcement decay after a forest fire in a Scots pine (Pinus sylvestris) protection forest. Forest Ecology and Management 2017; 400:339–352.

38. Lobet G., Pages L. and Draye X. A novel image-analysis toolbox enabling quantitative analysis of root system architecture. Plant Physiology 2008; 177:549–557.

39. Thanh T.X. and Thu D.H. Study on carbon accumulation capacity of the Acacia mangium plantation in Ngoc Thanh commune, Phuc Yen district, Vinh Phuc province, Vietnam. Proceeding of National Scientific Conference on Ecological and Biological Resources 2015; 6:1660–1666.

40. Laclau J.P, Almeida J.C.R., Goncallves J.L.M., Saint-Andrel L., Ventara M., Ranger J., et al. Influence of nitrogen and potassium fertilization on leaf lifespan and allocation of above-ground growth in Eucalyptus plantations. Tree Physiology 2009; 29:111–124. doi: 10.1093/treephys/tpn010 19203937

41. Zar J.H. Biostatistical Analysis. 5th ed. Pearson Education Ltd. DeKalb, IL, USA, 2010.

42. Rudhe J. Where has the fertilizer gone? Closing the nutrient budget for a eucalyptus fertilization experiment in southern China. Master Thesis. Swedish University of Agricultural Sciences; 2014.

43. Zhou W.J., Ji H., Zhu J., Zhang Y.P., Sha L.Q., Liu I.T., et al. The effects of nitrogen fertilization on N2O emissions from a rubber plantation. Scientific Report 2016; 6:28230.

44. Bah M., Husni M.H.A., Teh C.B.S., Rafii M.Y., Syed Omar S.R. and Ahmed O.H. Reducing Runoff loss of applied nutrients in oil palm cultivation using controlled-release fertilizers. Advances in Agriculture 2014; ID 285387: pp.9.

45. Carlson C.A., Fox T.R., Allen H.L., Albaugh T.J., Rubilar R.A. and Stape J.L. Growth responses of loblolly pine in the southeast united states to midrotation applications of nitrogen, phosphorus, potassium, and micronutrients. Forest Science 2014; 60:157–169.

46. Pritchett W.L. and Smith W.H. Fertilizer response in young slash pine stands. Soil Science Society of American Journal 1972; 36:660–663.

47. Raymond J.E., Fox T.R. and D. Strahm B.D. Understanding the fate of applied nitrogen in pine plantations of the Southeastern United States using 15N enriched fertilizers. Forests 2016; 7:270.

48. Mcintyre J. Fineroot responses to soil decompaction and amendment in red maple (Acer rubrum). Master Thesis. Clemson University; 2011.

49. Campbell J.J., Messier C. and Bauhus J. Does soil heterogeneity and compaction in ingrowth-cores affect growth and morphology of black spruce fine-roots?, Communication in Soil Science and Plant Analysis 2003; 33:1027–103.

50. O. Adu M., Chatot A., Wiesel L., Bennett M.J., Broadley M.R., White P.J. et al. A scanner system for high-resolution quantification of variation in root growth dynamics of Brassica rapa genotypes. Journal of Experimental Botany 2014; 65:2039–2048. doi: 10.1093/jxb/eru048 24604732

51. Balster N.J. and Marshall J.D. Eight-year responses of light interception, effective leaf area index, and stemwood production in fertilized stands of interior Douglas-fir (Pseudotsuga menziesii var. glauca). Canadian Journal of Forest Research 2000; 30:733–743.

52. Albaugh T.J., Allen H.L., Dougherty P.M., Kress L.W. and King J.S. Leaf area and above-and belowground growth responses of loblolly pine to nutrient and water additions. Forest Science 1998; 44:317–328.

53. Li H., Li M., Luo J., Cao X., Qu L., Gai Y., et al. fertilization has different effects on the growth, carbon and nitrogen physiology, and wood properties of slow-and fast-growing Populus species. Journal of Experimental Botany 2012; 63:6173–6185. doi: 10.1093/jxb/ers271 23028021

54. Maraseni T.N., Son H.L., Cockfield G., Duy H.V. and Nghia T.D. Comparing the financial returns from acacia plantations with different plantation densities and rotation ages in Vietnam. Forest Policy and Economic 2017; 83:80–87.

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2019 Číslo 11