Characterisation of a novel SCCmec VI element harbouring fusC in an emerging Staphylococcus aureus strain from the Arabian Gulf region


Autoři: Abiola Senok aff001;  Peter Slickers aff002;  Helmut Hotzel aff004;  Samar Boswihi aff005;  Sascha D. Braun aff002;  Darius Gawlik aff007;  Elke Müller aff002;  Anju Nabi aff008;  Rania Nassar aff001;  Hedda Nitschke aff009;  Annett Reissig aff002;  Antje Ruppelt-Lorz aff010;  Joseph Mafofo aff011;  Ali M. Somily aff012;  Edet Udo aff005;  Ralf Ehricht aff002;  Stefan Monecke aff002
Působiště autorů: College of Medicine, Mohammed Bin Rashid University of Medicine and Health Sciences, Dubai, United Arab Emirates aff001;  InfectoGnostics Research Campus Jena, Jena, Germany aff002;  Abbott (Alere Technologies GmbH), Jena, Germany aff003;  Friedrich-Loeffler-Institut (Federal Research Institute for Animal Health), Institute of Bacterial Infections and Zoonoses, Jena, Germany aff004;  Department of Microbiology, Faculty of Medicine, Kuwait University, Jabriya, Kuwait aff005;  Leibniz Institute of Photonic Technology (IPHT), Jena, Germany aff006;  PTC - Phage Technology Center GmbH, Bönen, Germany aff007;  Microbiology & Infection Control Unit, Pathology Department, Rashid Hospital, Dubai Health Authority, Dubai, United Arab Emirates aff008;  Department of Laboratory Medicine, Hospital Dresden-Neustadt, Dresden, Germany aff009;  Institute for Medical Microbiology and Hygiene, Medical Faculty "Carl Gustav Carus", Technische Universität Dresden, Dresden, Germany aff010;  Agiomix FZ-LLC, Dubai Science Park Warehouse Complex, Dubai, United Arab Emirates aff011;  Department of Pathology and Laboratory Medicine, College of Medicine, King Khalid University Hospital and King Saud University, Riyadh, Saudi Arabia aff012
Vyšlo v časopise: PLoS ONE 14(11)
Kategorie: Research Article
doi: 10.1371/journal.pone.0223985

Souhrn

Fusidic acid is a steroid antibiotic known since the 1960s. It is frequently used in topical preparations, i.e., ointments, for the treatment of skin and soft tissue infections caused by Staphylococcus aureus. There is an increasing number of methicillin-resistant S. aureus (MRSA) strains that harbour plasmid-borne fusB/far1 or fusC that is localised on SCC elements. In this study we examined a series of related CC30-MRSA isolates from the Arabian Gulf countries that presented with SCCmec elements and fusC, including a variant that—to the best of our knowledge—has not yet formally been described. It consisted of a class B mec complex and ccrA/B-4 genes. The fusidic acid resistance gene fusC was present, but contrary to the previously sequenced element of HDE288, it was not accompanied by tirS. This element was identified in CC30 MRSA from Kuwait, Saudi Arabia and the United Arab Emirates that usually also harbour the Panton-Valentin leukocidin (PVL) genes. It was also identified in CC8 and ST834 isolates. In addition, further CC30 MRSA strains with other SCCmec VI elements harbouring fusC were found to circulate in the Arabian Gulf region. It can be assumed that MRSA strains with SCCmec elements that include fusC have a selective advantage in both hospital and community settings warranting a review of the use of topical antibiotics and indicating the necessity of reducing over-the-counter sale of antibiotics, including fusidic acid, without prescription.

Klíčová slova:

Antibiotics – Antimicrobial resistance – Genetic elements – Methicillin-resistant Staphylococcus aureus – Sequence databases – Staphylococcus – Staphylococcus aureus – Saudi Arabia


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