Development of a quantitative PCR assay for the detection and enumeration of a potentially ciguatoxin-producing dinoflagellate, Gambierdiscus lapillus (Gonyaulacales, Dinophyceae)


Autoři: Anna Liza Kretzschmar aff001;  Arjun Verma aff001;  Gurjeet Kohli aff001;  Shauna Murray aff001
Působiště autorů: Climate Change Cluster (C3), University of Technology Sydney, Ultimo, New South Wales, Australia aff001;  ithree institute (i3), University of Technology Sydney, Ultimo, New South Wales, Australia aff002;  Alfred Wegener-Institut Helmholtz-Zentrum fr Polar- und Meeresforschung, Bremerhaven, Germany aff003
Vyšlo v časopise: PLoS ONE 14(11)
Kategorie: Research Article
doi: 10.1371/journal.pone.0224664

Souhrn

Ciguatera fish poisoning (CFP) is an illness contracted through the ingestion of seafood containing ciguatoxins. It is prevalent in tropical regions worldwide, including in Australia. Ciguatoxins are produced by some species of Gambierdiscus. Therefore, screening of Gambierdiscus species identification through quantitative PCR (qPCR), along with the determination of species toxicity, can be useful in monitoring potential ciguatera risk in these regions. In Australia, CFP is prevalent in tropical Queensland and increasingly in sub-tropical regions of Australia, but has a report rate of approximately 10%. Yet the identity, distribution and abundance of ciguatoxin producing Gambierdiscus spp. is largely unknown. In this study, we developed a rapid qPCR assay to quantify the presence and abundance of Gambierdiscus lapillus, a likely ciguatoxic species first described from Australia. We assessed the specificity and efficiency of the qPCR assay. The assay was tested on 25 environmental samples from the Heron Island reef in the southern Great Barrier Reef, a ciguatera endemic region, to determine the presence and patchiness of this species across samples from Chnoospora sp., Padina sp. and Sargassum sp. macroalgal hosts.

Klíčová slova:

Algae – Australia – DNA – DNA extraction – Islands – Polymerase chain reaction – Ribosomal RNA – Toxins


Zdroje

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