Anti-Alzheimer potential, metabolomic profiling and molecular docking of green synthesized silver nanoparticles of Lampranthus coccineus and Malephora lutea aqueous extracts

Autoři: Khayrya A. Youssif aff001;  Eman G. Haggag aff002;  Ali M. Elshamy aff003;  Mohamed A. Rabeh aff001;  Nagwan M. Gabr aff002;  Amany Seleem aff004;  M. Alaraby Salem aff005;  Ahmed S. Hussein aff005;  Markus Krischke aff006;  Martin J. Mueller aff006;  Usama Ramadan Abdelmohsen aff007
Působiště autorů: Department of Pharmacognosy, Faculty of Pharmacy, Modern University for Technology and Information, Cairo, Egypt aff001;  Department of Pharmacognosy, Faculty of Pharmacy, Helwan University, Cairo, Egypt aff002;  Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt aff003;  Department of Pharmacology, National Research Centre, Cairo, Egypt aff004;  Department of Pharmaceutical Chemistry, October University for Modern Sciences and Arts (MSA), Cairo, Egypt aff005;  Julius-von-Sachs-Institute of Biosciences, Biocenter, Pharmaceutical Biology, University of Würzburg, Würzburg, Germany aff006;  Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia, Egypt aff007;  Department of Pharmacognosy, Faculty of Pharmacy, Deraya University, Universities Zone, New Minia City, Minia, Egypt aff008
Vyšlo v časopise: PLoS ONE 14(11)
Kategorie: Research Article
doi: 10.1371/journal.pone.0223781


The green synthesis of silver nanoparticles (SNPs) using plant extracts is an eco-friendly method. It is a single step and offers several advantages such as time reducing, cost-effective and environmental non-toxic. Silver nanoparticles are a type of Noble metal nanoparticles and it has tremendous applications in the field of diagnostics, therapeutics, antimicrobial activity, anticancer and neurodegenerative diseases. In the present work, the aqueous extracts of aerial parts of Lampranthus coccineus and Malephora lutea F. Aizoaceae were successfully used for the synthesis of silver nanoparticles. The formation of silver nanoparticles was early detected by a color change from pale yellow to reddish-brown color and was further confirmed by transmission electron microscope (TEM), UV–visible spectroscopy, Fourier transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS), X-ray diffraction (XRD), and energy-dispersive X-ray diffraction (EDX). The TEM analysis of showed spherical nanoparticles with a mean size between 12.86 nm and 28.19 nm and the UV- visible spectroscopy showed λmax of 417 nm, which confirms the presence of nanoparticles. The neuroprotective potential of SNPs was evaluated by assessing the antioxidant and cholinesterase inhibitory activity. Metabolomic profiling was performed on methanolic extracts of L. coccineus and M. lutea and resulted in the identification of 12 compounds, then docking was performed to investigate the possible interaction between the identified compounds and human acetylcholinesterase, butyrylcholinesterase, and glutathione transferase receptor, which are associated with the progress of Alzheimer’s disease. Overall our SNPs highlighted its promising potential in terms of anticholinesterase and antioxidant activity as plant-based anti-Alzheimer drug and against oxidative stress.

Klíčová slova:

Alzheimer's disease – Antioxidants – Brain diseases – Glutathione – Metabolomics – Nanoparticles – Serine proteases – Silver


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