Molecular diet analysis of Anguilliformes leptocephalus larvae collected in the western North Pacific


Autoři: Seinen Chow aff001;  Nobuharu Inaba aff001;  Satoshi Nagai aff001;  Hiroaki Kurogi aff001;  Yoji Nakamura aff001;  Takashi Yanagimoto aff001;  Hideki Tanaka aff003;  Daisuke Hasegawa aff004;  Taiga Asakura aff005;  Jun Kikuchi aff005;  Tsutomu Tomoda aff006;  Taketoshi Kodama aff001
Působiště autorů: National Research Institute of Fisheries Science, Japan Fisheries Research and Education Agency, Kanazawa, Yokohama, Japan aff001;  Civil Engineering Research Institute for Cold Region, Public Works Research Institute, Sapporo, Hokkaido, Japan aff002;  Aquaculture Research Institute, Kindai University, Higashimuro, Wakayama, Japan aff003;  Tohoku National Fisheries Research Institute, Japan Fisheries Research and Education Agency, Shiogama, Miyagi, Japan aff004;  RIKEN Center for Sustainable Resource Science, Tsurumi, Yokohama, Japan aff005;  Shibushi Station, National Research Institute of Aquaculture, Japan Fisheries Research and Education Agency, Shibushi, Kagoshima, Japan aff006
Vyšlo v časopise: PLoS ONE 14(11)
Kategorie: Research Article
doi: 10.1371/journal.pone.0225610

Souhrn

Natural diets of leptocephalus larvae have been enigmatic. In this study, we collected DNA samples from the gut contents and body surface of leptocephali belonging to the five Anguilliform families (Anguillidae, Chlopsidae, Congridae, Muraenidae, and Serrivomeridae) from the northwest Pacific and performed next-generation 18S rDNA sequencing. Wide variety of eukaryotes was detected in both samples, from which eight eukaryotic groups (jellyfish, conoid parasite, tunicate, copepod, krill, segmented worm, fungi, and dinoflagellate) were selected on the basis of abundance. All groups except conoid parasites were common in both the samples. Cnidarian 18S rDNA reads were the most abundant in both the samples; however, the number of samples having cnidarian reads and the read counts were significantly higher in the body surface scraping samples than in the gut content samples, regardless of careful rinsing of the body surface. These results indicate that the cnidarian DNAs are most likely found because of cross contamination from the body surface and/or environment. 18S rDNA read counts of copepod and tunicate in the gut contents were greater than or comparable with those in the body surface scraping samples, which may correspond to the previous observations of fecal pellets and larvacean houses in the leptocephali gut. Thus, the present study supports previous implications that leptocephali utilize detritus materials, so called marine snow.

Klíčová slova:

Copepods – Dinoflagellates – Eels – Eukaryota – Larvae – Polymerase chain reaction – Jellyfish – Specimen scraping


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2019 Číslo 11