Comparative studies of two generations of NanoString nCounter System

Autoři: Lianbo Yu aff001;  Sagar Bhayana aff002;  Naduparambil K. Jacob aff002;  Paolo Fadda aff003
Působiště autorů: Center for Biostatistics, Department of Biomedical Informatics, The Ohio State University, Columbus, Ohio, United States of America aff001;  Department of Radiation Oncology, The Ohio State University, Columbus, Ohio, United States of America aff002;  Genomics Shared Resource, Comprehensive Cancer Center, The Ohio State University, Columbus, Ohio, United States of America aff003
Vyšlo v časopise: PLoS ONE 14(11)
Kategorie: Research Article
doi: 10.1371/journal.pone.0225505


The NanoString nCounter System has been widely used in basic science and translational science research for the past decade. The System consists of two instruments: the PrepStation and the Digital Analyzer, and both have been continuously improved with evolving technologies. A great amount of research data have been generated at multiple research laboratories with the employment of different generations of the System. With the need of integrating multiple datasets, researchers are interested to know whether signals are comparable between different generations of the System. Toward this end, we designed a profiling study to compare performance of two generations of the NanoString nCounter System using a common set of biological samples. Using graphical tools and statistical models, we found that two different generations of NanoString nCounter System produced equivalent signals and signal deviations are in the range of random background noises for the medium-high expression levels.

Klíčová slova:

Analysis of variance – Bionanotechnology – Curve fitting – Experimental design – Hierarchical clustering – MicroRNAs – Nanotechnology – RNA isolation


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Článek vyšel v časopise


2019 Číslo 11