Preliminary evidences of the presence of extracellular DNA single stranded forms in soil

Autoři: Shamina Imran Pathan aff001;  Paola Arfaioli aff001;  Maria Teresa Ceccherini aff001;  Judith Ascher-Jenull aff002;  Giacomo Pietramellara aff001
Působiště autorů: Department of Agri-food, Environmental, Forestry Science and Technology (DAGRI), University of Florence, Piazzale delle Cascine, Florence, Italy aff001;  Institute of Microbiology, University of Innsbruck, Innsbruck, Austria aff002
Vyšlo v časopise: PLoS ONE 15(1)
Kategorie: Research Article


The relevance of extracellular DNA (eDNA) in the soil ecosystem is becoming more and more evident to the scientific community by the progressive discovery of functions accompanying to natural gene transformation. However, despite the increased number of published articles dedicated to eDNA in soil, so far only few are focused on its single stranded form (eDNAss). The present paper is the first to investigate the quantitative relevance of eDNAss in the total soil eDNA pool, discriminating between its linear (eDNAssl) and circular (eDNAssc) forms and the respective weakly (wa) and tightly (ta) adsorbed fractions. The results showed the prevalence of eDNAss and its linear form in both the total soil eDNA pool and its wa and ta fractions. Both of the eDNAss fractions (linear and circular) were characterized by small fragments.

Klíčová slova:

Adsorption – Analysis of variance – DNA – DNA extraction – Elution – Gene pool – Soil ecology – Qubits


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