Models of protein production along the cell cycle: An investigation of possible sources of noise


Autoři: Renaud Dessalles aff001;  Vincent Fromion aff002;  Philippe Robert aff003
Působiště autorů: Dept. of Biomathematics, UCLA, Los Angeles, CA, United States of America aff001;  MaIAGE, INRA, Université Paris-Saclay, Jouy-en-Josas, France aff002;  INRIA de Paris, Paris, France aff003
Vyšlo v časopise: PLoS ONE 15(1)
Kategorie: Research Article
doi: 10.1371/journal.pone.0226016

Souhrn

In this article, we quantitatively study, through stochastic models, the effects of several intracellular phenomena, such as cell volume growth, cell division, gene replication as well as fluctuations of available RNA polymerases and ribosomes. These phenomena are indeed rarely considered in classic models of protein production and no relative quantitative comparison among them has been performed. The parameters for a large and representative class of proteins are determined using experimental measures. The main important and surprising conclusion of our study is to show that despite the significant fluctuations of free RNA polymerases and free ribosomes, they bring little variability to protein production contrary to what has been previously proposed in the literature. After verifying the robustness of this quite counter-intuitive result, we discuss its possible origin from a theoretical view, and interpret it as the result of a mean-field effect.

Klíčová slova:

Biochemical simulations – Cell cycle and cell division – DNA replication – DNA transcription – Messenger RNA – Protein expression – Ribosomes – Simulation and modeling


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