#PAGE_PARAMS# #ADS_HEAD_SCRIPTS# #MICRODATA#

Optimization of tissue sampling for Borrelia burgdorferi in white-footed mice (Peromyscus leucopus)


Autoři: Sonya G. Zawada aff001;  Michael E. von Fricken aff003;  Thomas A. Weppelmann aff004;  Masoumeh Sikaroodi aff005;  Patrick M. Gillevet aff005
Působiště autorů: Department of Environmental Science & Public Policy, George Mason University, Fairfax, Virginia, United States of America aff001;  Department of Biology, University of Mary Washington, Fredericksburg, Virginia, United States of America aff002;  Department of Global and Community Health, George Mason University, Fairfax, Virginia, United States of America aff003;  Herbert Wertheim College of Medicine, Florida International University, Miami, Florida, United States of America aff004;  Department of Biology, George Mason University, Fairfax, Virginia, United States of America aff005
Vyšlo v časopise: PLoS ONE 15(1)
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pone.0226798

Souhrn

Peromyscus leucopus (the white-footed mouse) is a known reservoir of the Lyme disease spirochete Borrelia burgdorferi. Sampling of white-footed mice allows for year-round B. burgdorferi surveillance as well as opportunities to establish the diversity of the different variants in a geographic region. This study explores the prevalence of B. burgdorferi infections in the tissues of white-footed mice, investigates the correlations between B. burgdorferi infected tissues, and determines the optimum field methods for surveillance of B. burgdorferi in P. leucopus. A total of 90 mice and 573 tissues (spleen, liver, ear, tongue, tail, heart, and kidney) were screened via nested PCR for B. burgdorferi infections. A large number of infections were found in the 90 mice as well as multiple infections within individual mice. Infections in a single mouse tissue (spleen, liver, ear, tongue and tail) were predictive of concurrent infection in other tissues of the same mouse at a statistically significant level. Ear tissue accounted for 68.4% of detected infections, which increased to 78.9% of the infected mice with the inclusion of tail samples. The use of ear punch or tail snip samples (used individually or in tandem) have multiple advantages over current Lyme disease ecological studies and surveillance methodologies, including lower associated costs, minimization of delays, year-round B. burgdorferi testing opportunities, as well as longitudinal monitoring of B. burgdorferi in defined geographic regions. In the absence of an effective vaccine, personal prevention measures are currently the most effective way to reduce Lyme disease transmission to humans. Thus, the identification and monitoring of environmental reservoirs to inform at-risk populations remains a priority. The sampling methods proposed in this study provide a reasonable estimate of B. burgdorferi in white-footed mice in a timely and cost-effective manner.

Klíčová slova:

Borrelia burgdorferi – Ear infections – Heart – Infectious disease surveillance – Kidneys – Lyme disease – Spleen – Tongue


Zdroje

1. Steere A, Coburn J, Glickstein L. The emergence of Lyme disease. J Clin Invest. 2004;113(8): 1093–101. doi: 10.1172/JCI21681 15085185

2. Kugeler K, Farley G, Forrester J, Mead P. Geographic Distribution and Expansion of Human Lyme Disease, United States. Emerg Infect Dis. 2015;21(8):1455–7. doi: 10.3201/eid2108.141878 26196670

3. Khatchikian C., Prusinski M, Stone M, Backenson P, Wang I, Foley E, et al. Recent and Rapid Population Growth and Range Expansion of the Lyme Disease Tick Vector, Ixodes Scapularis , in North America: POPULATION AND RANGE EXPANSION OF TICKS. Evolution 2015; 69 (7): 1678–89. doi: 10.1111/evo.12690 26149959

4. Peavey C and Lane R. Transmission of Borrelia Burgdorferi by Ixodes Pacificus Nymphs and Reservoir Competence of Deer Mice (Peromyscus Maniculatus) Infected by Tick-Bite. J Parasitology. 1995;81(2):175–78.

5. Shapiro E and Gerber M. Lyme Disease. Clin Infect Dis. 2000;31(2): 533–42. doi: 10.1086/313982 10987718

6. Nadelman R and Wormser G. Lyme borreliosis. Lancet. 1998;352:557–565. doi: 10.1016/S0140-6736(98)01146-5 9716075

7. Cartter M, Lynfield R, Feldman K, Hook S, Hinckley A. Lyme disease surveillance in the United States: Looking for ways to cut the Gordian knot. Zoonoses Public Hlth. 2018; 65(2):227–229.

8. Anderson J, Johnson R and Magnarelli L. Seasonal Prevalence of Borrelia Burgdorferi in Natural Populations of White-Footed Mice, Peromyscus Leucopus. J Clin Micro. 1987; 25(8):1564–66.

9. Hofmeister E and Childs J. Burgdorferi by PCR, but not by culture in naturally infected peromyscus leucopus. J Wildlife Dis. 1995;31(3):345–351.

10. Wang I, Dykhuizen D, Qiu W, Dunn J, Bosler E, Luft B. Genetic Diversity of ospC in a Local Population of Borrelia Burgdorferi Sensu Stricto. Genetics. 1999;151(1): 15–30. 9872945

11. Rutz H, Wee S and Feldman K. Characterizing Lyme Disease Surveillance in an Endemic State. Zoonoses Public Hlth. 2018;65(2):247–253.

12. Stafford K, Cartter M, Magnarelli L, Ertel S, Mshar P. Temporal Correlations between Tick Abundance and Prevalence of Ticks Infected with Borrelia Burgdorferi and Increasing Incidence of Lyme Disease. J Clin Micro. 1997;36(5): 1240–44.

13. Shen A, Mead P and Beard C. The Lyme Disease Vaccine—A Public Health Perspective. Clin Infect Dis. 2011;52(3): 247–252.


Článek vyšel v časopise

PLOS One


2020 Číslo 1
Nejčtenější tento týden
Nejčtenější v tomto čísle
Kurzy

Zvyšte si kvalifikaci online z pohodlí domova

Svět praktické medicíny 1/2024 (znalostní test z časopisu)
nový kurz

Koncepce osteologické péče pro gynekology a praktické lékaře
Autoři: MUDr. František Šenk

Sekvenční léčba schizofrenie
Autoři: MUDr. Jana Hořínková

Hypertenze a hypercholesterolémie – synergický efekt léčby
Autoři: prof. MUDr. Hana Rosolová, DrSc.

Význam metforminu pro „udržitelnou“ terapii diabetu
Autoři: prof. MUDr. Milan Kvapil, CSc., MBA

Všechny kurzy
Kurzy Podcasty Doporučená témata Časopisy
Přihlášení
Zapomenuté heslo

Zadejte e-mailovou adresu, se kterou jste vytvářel(a) účet, budou Vám na ni zaslány informace k nastavení nového hesla.

Přihlášení

Nemáte účet?  Registrujte se

#ADS_BOTTOM_SCRIPTS#