Influence of inflammasome NLRP3, and IL1B and IL2 gene polymorphisms in periodontitis susceptibility


Autoři: Josiane Bazzo de Alencar aff001;  Joana Maira Valentini Zacarias aff001;  Patrícia Yumeko Tsuneto aff001;  Victor Hugo de Souza aff001;  Cléverson de Oliveira e Silva aff002;  Jeane Eliete Laguila Visentainer aff001;  Ana Maria Sell aff001
Působiště autorů: Department of Clinical Analysis and Biomedicine, Post-Graduation Program in Biosciences and Physiophatology, State University of Maringá, Maringá, Paraná, Brazil aff001;  Department of Dentistry, State University of Maringá, Maringá, Paraná, Brazil aff002;  Department of Basic Health Sciences, State University of Maringá, Maringá, Paraná, Brazil aff003
Vyšlo v časopise: PLoS ONE 15(1)
Kategorie: Research Article
doi: 10.1371/journal.pone.0227905

Souhrn

The pathogenesis of periodontitis (PD) involves several molecules of the immune system that interact in a network to eliminate the periodontopathogens, yet, they contribute to periodontal tissue destruction. The different mechanisms that lead to periodontal tissue damage are not clear. Despite this, immune response genes have been related to the development of PD previously, such as those involved in inflammasomes which are multiprotein complexes and cytokines including Interleukin-1. The aim of the study was to evaluate the polymorphisms in NLRP3 inflammasome, cytokine and receptor of cytokines genes in the development of periodontitis. This case-control study was conducted in 186 patients with PD (stage II and III and grade B) and 208 controls (localized gingivitis and periodontally healthy individuals). Genotyping was performed using PCR-RFLP for the SNP rs4612666 in NLRP3 and using PCR-SSP for IL1A, IL1B, IL1R, IL1RN, IL4RA, INFG, TGFB1, TNF, IL2, IL4, IL6, and IL10. Cytokine serum levels were measured using Luminex technology. SNPStats and OpenEpi software were used to perform statistical analysis. The higher frequencies of NLRP3 T/C and IL1B -511 T/T genotypes and IL2 (+166, -330) GT haplotype were observed in patients with PD compared to controls. The SNPs in NLRP3, IL1R +1970, IL6–174, TNF -308, IL2 +166 and -330, TGFB1 +869 and +915, IL4RA +1902, IL4–1098 and -590 were associated to PD in men. In conclusion, polymorphisms in NLRP3, IL1B and IL2 genes were associated to PD susceptibility. Men carrying the NLRP3, IL1R, IL6, TNF, IL2, TGFB1, IL4RA and IL4 polymorphisms had greater susceptibility than women for developing PD.

Klíčová slova:

Brazil – Cytokines – Haplotypes – Immune response – Inflammasomes – Periodontitis – Smoking habits – Variant genotypes


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