PCR for the detection of pathogens in neonatal early onset sepsis


Autoři: Clarissa Oeser aff001;  Marcus Pond aff002;  Philip Butcher aff002;  Alison Bedford Russell aff003;  Philipp Henneke aff004;  Ken Laing aff002;  Timothy Planche aff002;  Paul T. Heath aff001;  Kathryn Harris aff005
Působiště autorů: Paediatric Infectious Diseases, Institute of Infection and Immunity, St George’s, University of London, London, United Kingdom aff001;  Molecular Microbiology, Institute of Infection and Immunity, St George’s, University of London, London, United Kingdom aff002;  Neonatology, Birmingham Women's NHS Foundation Trust, Birmingham, United Kingdom aff003;  Pediatric Infectious Disease and Rheumatology, University Medical Center Freiburg, Freiburg, Germany aff004;  Microbiology, Virology and Infection Control, Great Ormond Street Hospital NHS Foundation Trust, London, United Kingdom aff005
Vyšlo v časopise: PLoS ONE 15(1)
Kategorie: Research Article
doi: 10.1371/journal.pone.0226817

Souhrn

Background

A large proportion of neonates are treated for presumed bacterial sepsis with broad spectrum antibiotics even though their blood cultures subsequently show no growth. This study aimed to investigate PCR-based methods to identify pathogens not detected by conventional culture.

Methods

Whole blood samples of 208 neonates with suspected early onset sepsis were tested using a panel of multiplexed bacterial PCRs targeting Streptococcus pneumoniae, Streptococcus agalactiae (GBS), Staphylococcus aureus, Streptococcus pyogenes (GAS), Enterobacteriaceae, Enterococcus faecalis, Enterococcus faecium, Ureaplasma parvum, Ureaplasma urealyticum, Mycoplasma hominis and Mycoplasma genitalium, a 16S rRNA gene broad-range PCR and a multiplexed PCR for Candida spp.

Results

Two-hundred and eight samples were processed. In five of those samples, organisms were detected by conventional culture; all of those were also identified by PCR. PCR detected bacteria in 91 (45%) of the 203 samples that did not show bacterial growth in culture. S. aureus, Enterobacteriaceae and S. pneumoniae were the most frequently detected pathogens. A higher bacterial load detected by PCR was correlated positively with the number of clinical signs at presentation.

Conclusion

Real-time PCR has the potential to be a valuable additional tool for the diagnosis of neonatal sepsis.

Klíčová slova:

Antibiotics – Bacterial pathogens – Blood – Enterobacteriaceae – Neonatal sepsis – Neonates – Pneumococcus – Polymerase chain reaction


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